HPLC法测定五维B颗粒中维生素B_1的含量

目的:建立以高效液相色谱法测定五维B颗粒中维生素B1含量的方法。方法:色谱柱为YWG-C18,流动相为1%冰醋酸溶液(含0.005mol.L-1庚烷磺酸钠)-甲醇-乙腈(65∶21∶14),流速为1.0mL.min-1,检测波长为254nm,进样量为5μL。结果:维生素B1检测浓度的线性范围为0.1～0.6mg.mL-1(r=0.9997);平均回收率为100.3%,RSD=0.36%。结论:本方法简便、快速、准确、专属性强,可用于该制剂中维生素B1的含量测定。

Determination of VB1 in Five Vitamin-Granules by HPLC

OBJECTIVE:To establish HPLC method for the determination of VB1 in five vitamin-granules.METHODS:YWG-C18 column was used.The mobile phase was dilute glacial acetic acid containing 0.005mol· L-1 sodium heptanesulfonate-methanol-acetonitrile(65∶ 21∶ 14)with a flow rate of 1.0mL· min-1.The detection wavelength was set at 254nm and the sample size was 5μ L.RESULTS:The calibration curve of VB1 was linear in the range of 0.1～ 0.6mg· mL-1(r=0.999 7).The average recovery was 100.3%(RSD=0.36%).CONCLUSION:The method was proved to be simple,rapid,accurate and specific,and it can be used for the quantitative determination of VB1 in five vitamin-granules.