山东泰安地区TTV检测和部分基因克隆及序列测定

目的 探讨山东非甲-庚肝炎和肝癌患者输血传播病毒(TTV)感染状况和基因变异情况。方法 应用逆转录聚合酶链反应(PCR)检测26例山东泰安地区非甲-庚型肝炎和12例肝细胞癌(HCC)患者血清中TTVDNA，并对阳性扩增的产物利用PCR技术片段直接克隆和测序，分析其基因变异的情况。结果 26例非甲-庚型肝炎中11例TTVDNA阳性(42．3％)。对其中两株(TTVSD4、TTVSD5)部分基因克隆测序，并与日本株(AB008394)相比较，其核苷酸序列同源性分别为99．9％和100％。而12例肝细胞癌患者中3例TTVDNA阳性(25．0％)，对其中一株(TTVSD6)部分基因克隆与测序，与日本株(AB008394)相比较，其核苷酸序列同源性为99％；TTV山东泰安三株问核苷酸同源性均为99％。结论 研究证实山东泰安地区非甲～庚型肝炎和肝细胞癌患者中存在着较高TTV感染，TTV感染可能具有嗜肝性，而且可能与肝功能损害有关，是引起非甲～庚型肝炎的重要病原。

Detection of transfusion transmitted virus（TTV）and partial gene cloning and sequencing in the Taian areas of Shandong

Objective To understand the prevalence of TTV infection and genetic variation of TTV isolated in Shandong Province.Methods TTVDNA were amplified and detected by PCR from sera of patients with NonA-G hepatitis and hepatic cancer. The PCR products were cloned and sequenced.Results TTVDNA was positive in 11 of 26 patients(42.3%) with NonA-G hepatitis. Partial gene of two TTV isolated(TTVSD4,TTVSD5) was cloned, sequenced and compared with known sequence of TTV isolates in Japan(ABOO8394) reported by Okamoto. The nucleotide homology were 99% and 100%. TTVDNA was positive in 3 of 12 patients (25%) with hepatic cancer. Partial gene of a TTV isolated (TTVSD6) was cloned, sequenced and compared with known sequence of TTV isolated in Japan(ABOO8394) reported by Okamoto. The nucleotide homology was 99%..Conclusions The ratio of TTV infection is high in the Taian areas of Shandong. TTV might be hepatotropic, and it probably correlated with the impairment of liver function. So it is likely to be the pathogen of NonA-G hepatitis.