补肾方对流产大鼠胎盘超微结构的影响

为了在组织形态学上探讨补肾方药对流产的治疗作用，寻找其作用的形态学基础，对澳隐亭致流产SD大鼠模型，以补肾方高、低剂量为治疗组、黄体酮肌注为阳性对照组，于孕12d处死，取下胎盘组织以透射电镜观察各组超徽结构。结果发现与正常组相比，模型组的胎盘组织出现下列明显的超微结构改变：在合体滋养层，游离面的血窦密度明显减小；数量较多的线粒体肿胀；粗面内质网池扩张，膜上核糖体有脱颗粒现象；基底膜弯曲、增厚；在基底膜内或附近常见电子致密球状颗粒；微血管内皮增厚，伴有微绒毛样突起。补肾中药组和黄体酮组滑面内质网增加，呈扩张状态，线粒体丰富，胞质内有大量脂滴，其它细胞器基本正常。结果提示，溴隐亭引起大鼠胎盘结构改变，影响其进行物质交换和分泌激素的功能，从而引起流产的发生。补肾中药作用后可逆转流产胎盘超微结构的形态学改变，使其向有利于甾体激素合成的方向转化，起到维持妊娠的作用。

Effect of Kidney Replenishing Herbs on Placenta Ultrastructure in Abortion Rat

To investigate the placental pathological changes after treatment of replenishing kidney formula , early abortion rat model was established by Bromocriptine at 6-8 gestation day ,the model rats were treated with high and low dosage of the herbs and the progesterone as the positive control, the rats were sacrificed at 12 gestation day and the ultrastructure of the placenta were observed by electron microscope .The results find that compared with the normal control the basement membrane of thetrophoblastic epithelium of model group was thickened and tortuous, within the basement membrane there were lots of dense microspherical particles, mitochondrial showed coagulating and vacant, the cisternae of the rough endoplasmic reticulum(rER) were dilated and contained flocky material and also the degranulation of ribosomes on the rER were frequently found, the capillary endothelium was thicked with microvilli-like projections. In herbs and progesterone groups, smooth endoplasmic reticulum(sER) increased and dilated, there were plenty of mitochondria and some lipid droplet in cytoplasm. organell were normal mainly. The results suggested that the Bromocriptine could induce changes of placental structure and influenced placental normal function. Kidney replenishing herbs could reverse morphological alteration of ultrastrcture in abortion placenta , and converted to benefit in hormone synthesis, finally achieved the aim to prevent abortion.