| 胰高血糖素样肽-2联合谷氨酰胺对放射损伤后肠黏膜细胞凋亡的影响 |
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| 引用本文： | 张坚,朱维铭,张伟,李宁,黎介寿.胰高血糖素样肽-2联合谷氨酰胺对放射损伤后肠黏膜细胞凋亡的影响[J].肠外与肠内营养,2008,15(2):73-76. |
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| 作者姓名： | 张坚 朱维铭 张伟 李宁 黎介寿 |
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| 作者单位： | 南京大学医学院临床学院(南京军区南京总医院)解放军普通外科研究所,江苏南京,2100002 |
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| 摘 要： | 目的: 探讨胰高血糖素样肽-2(GLP-2)联合应用Gln对放射损伤后肠黏膜细胞凋亡的影响. 方法: 将50只小鼠随机分为正常组、放射组、GLP-2处理组(GLP-2组)、Gln处理组(Gln组)和Gln和GLP-2联合处理组(Gln/GLP-2组),每组10只.实验期为7 d.GLP-2组于实验第1～6天腹腔注射GLP-2;Gln组于实验第1～6天灌饲Gln溶液;Gln/GLP-2组给予Gln和GLP-2联合处理,方法同上.在实验第4天,各组小鼠(除正常组)接受11Gy γ-射线腹部照射.实验第7天取小肠标本,行TUNEL法检测原位细胞凋亡,并用荧光法检测肠上皮细胞Caspase-3 酶活性. 结果: GLP-2组的肠黏膜细胞凋亡指数和Caspase-3酶活性与放射组相比无显著差异,而Gln组较放射组明显降低(P<0.01);Gln/GLP-2组的细胞凋亡指数和Caspases-3 酶活性较Gln组进一步降低(P<0.01),与正常组相比无显著差异. 结论:GLP-2和Gln联合应用能明显抑制放射损伤后肠黏膜细胞凋亡.
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| 关 键 词： | 胰高血糖素样肽-2 谷氨酰胺 放射损伤 小肠 细胞凋亡 |
| 文章编号： | 1007-810X(2008)02-0073-04 |
| 修稿时间： | 2007年11月1日 |
Effect of combined treatment with glucagon like peptide-2 and glutamine on radiation-induced enteric epithelium apoptosis |
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| ZHANG Jian,ZHU Wei-ming,ZHANG Wei,LI Ning,LI Jie-shou.Effect of combined treatment with glucagon like peptide-2 and glutamine on radiation-induced enteric epithelium apoptosis[J].Parenteral & Enteral Nutrition,2008,15(2):73-76. |
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| Authors: | ZHANG Jian ZHU Wei-ming ZHANG Wei LI Ning LI Jie-shou |
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| Institution: | ZHANG Jian,ZHU Wei-ming,ZHANG Wei,LI Ning,LI Jie-shou(Research Institute of General Surgery,Clinical School of Medicine,Nanjing University/Nanjing General Hospital of Nanjing Military Comm,,PLA,Nanjing 210002,Jiangsu,China) |
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| Abstract: | Objective: To explore the effect of combined treatment with glucagon like peptide-2(GLP-2) and glutamine(Gln) on radiation-induced enteric epithelium apoptosis.Methods: Fifty B6 mice were randomly divided into five groups(n=10): Control group,Rad group,GLP-2 group,Gln group and Gln/GLP-2 group.All the mice except normal group received abdominal radiation on day 4,and were sacrificed on day 7.The apoptosis of intestinal epithelial cells and activity of Caspase-3 were detected by TUNEL and fluorescence method... |
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| Keywords: | Glucagon-like peptide 2 Glutamine Radiation Injuries Intestine Cell apoptosis |
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