正交试验优化健胃灵颗粒的提取工艺

目的:对健胃灵颗粒的提取工艺进行优化。方法:用乙醇作溶剂提取处方中7种药材的醇溶性成分,按正交表L9(34)安排试验,以芍药苷含量和浸膏得率的加权和为定量指标,对影响醇提取效率的4个因素进行考察;再加入其余药材,用水煎煮法提取水溶性成分。结果:醇溶性成分提取的最佳工艺为:8倍量60%乙醇、浸泡45min、加热回流提取3次(每次1.5h);水溶性成分提取的最佳工艺为:10倍量水、煎煮2次(每次1.5h)。结论:本法能最大限度地提取处方中各种药材的有效成分,可为健胃灵颗粒的工业化生产提供理论依据。

Optimizing the Extraction Technique for Jianweiling Granules by Orthogonal Test

OBJECTIVE:To optimize the technique for extraction of Jianweiling granules.METHODS:Ethanol was used as solution to extract the seven ethanol dissolvable ingredients in the formula.L9(34)table was used to examine the effects of the 4 factors,and the weighted sum of paeoniflorin content and the dried extract quantity were determined by orthogonal test.Other water dissolvable ingredients were extracted by liquid boiling & decocting.RESULTS:The optimum technique for extraction of ethanol dissolvable ingredients is:8-fold 60% ethanol solution soaked for 45 min,and extracted by heating reflux for 3 times,1.5h per time;the optimum technique for extraction of water dissolvable ingredients is:10-fold water,boil-decocted for 2 times,1.5h per time.CONCLUSION:The method can maximize extraction of the effective ingredients in all medicinal materials of the formula,and can provide theoretic support for industrialization of Jianweiling granules.