骨髓增生异常综合征发病机制探讨

目的 :探讨骨髓增生异常综合征 (MDS)的发病机制。方法 :采用造血祖细胞体外培养技术 ,观察 40例MDS患者骨髓细胞粒 巨噬细胞集落形成单位 (GM CFU)体外生长情况及患者血清、骨髓细胞对正常人造血祖细胞体外增殖作用的影响。结果 :MDS中的难治性贫血 (RA) 74%为造血干细胞缺损 (HSCD) ;原始细胞增多的难治性贫血 (RAEB)、转化中的原始细胞增多的难治性贫血 (RAEB T)、慢性粒单细胞白血病 (CMML) 10 0 %为免疫介导型 (IC) ;环形铁粒幼红细胞增多的难治性贫血 (RAS)仅 2例 ,造血微环境缺陷 (HMED)及IC型各 1例。结论 :RA、RAS与RAEB、RAEB T、CMML患者可能是两种本质不同的群体 ,研究MDS的发病机制对克服治疗上的盲目性 ,提高疗效、判断预后具有重要意义

Effect of cytokines on proliferation and collagen synthesis by bovine lens epithelial cells in vitro

Aim: To explore the pathogenetic mechanisms of myelodisplastic syndrome (MDS). Methods: Bone marrow (BM) cells were cultured for granulocyte macrophage colony forming unit(GM CFU) to evaluate the influence of bone marrow cells and sera of 40 patients with MDS on hemoblastosis of Normal BM stem cells in vitro. Results: The number of GM CFU in patients with MDS were lower than that of normal, 74% of RA were the hemotopoitic stem cell defective (HSCD), the rest were immune conductive (IC); 1 out of 2 cases of RAS was HSCD, another was hemopoitic microenvironment defective (HMED). The sera and BM cells of RAEB?RAEB T?CMML could suppress the growth of GM CFU from normal BM cells (100%),owe to the type of IC. Conclusion: The pathogenetic mechanisms of MDS are complex,Study on mechanisms of MDS has important clinical significance in overcoming the blindness of treatment, increasing therapeutic efficacy and judging prognosis of MDS.