| 消咳喘片中金丝桃苷的含量测定 |
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| 引用本文： | 杜月莲,李青山.消咳喘片中金丝桃苷的含量测定[J].世界中西医结合杂志,2009,4(11):788-789. |
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| 作者姓名： | 杜月莲 李青山 |
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| 作者单位： | 1. 山西医科大学药学院,山西,太原,030001;山西生物应用职业技术学院,山西,太原,030031
2. 山西医科大学药学院,山西,太原,030001 |
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| 摘 要： | 目的建立消咳喘片中金丝桃苷的含量测定方法。方法采用HypersilC18（4．6mm×250mm，5μm）色谱柱，甲醇：0．5％的磷酸水溶液（45：55，三乙胺调pH至3）为流动相，流速为0．8mL／min，柱温为30％，检测波长为355nm。结果金丝桃苷在0．12～1．92μg范围内线性关系良好，平均回收率为100．56％，RSD=2．10％。结论所建立的方法准确、灵敏、重现性好，可用于消咳喘片的质量控制。
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| 关 键 词： | 消咳喘片 金丝桃苷 HPLC 含量测定 |
Content Determination of Hyperin in Xiaokechuan Tablets |
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| DU Yue-lian,LI Qing-shan.Content Determination of Hyperin in Xiaokechuan Tablets[J].World Journal Of Integrated Traditional and Wesrern Medicine,2009,4(11):788-789. |
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| Authors: | DU Yue-lian LI Qing-shan |
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| Institution: | DU Yue- lian, LI Qing- shah ( 1. Pharmaceutical School, Shanxi Medical University,Taiyuan Shanxi 030001 ;2. Shanxi Biological Applica- tion Vocational School ,Taiyuan Shanxi 030031 ) |
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| Abstract: | Objective To establish the determination method of hyperin content in xiaokechuanhuan tablets. Methods Hypersil C is （4.6 mm×250mm,5μm）column was used, with mobile phase of acetic acid aqueous solution at the ratio of methanol： 0.5% （45： 55, triethylamine was adjusted to be pH 3 ） ,0.8 mL/min in the flow rate, at 30℃ and 355 nm detection wavelength. Results The linear relation of hyperin was good in the range from 0.12 tol. 92 μg. The average recovery was 100.56% ,RSD =2.10%. Conclusion The method established is accurate, sensitive and good in reproducibility. It can be applied to the quality control of xiaokechuan tablets. |
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| Keywords: | HPLC |
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