肿瘤坏死因子相关凋亡诱导配体通过促进死亡相关蛋白3表达抑制人胃癌细胞株的生长

目的：观察肿瘤坏死因子相关凋亡诱导配体（TRAIL）对人胃癌细胞株的生长抑制作用及其作用前、后人胃癌细胞株中死亡相关蛋白3（DAP3）表达情况，探讨DAP3在TRAIL抑制人胃癌细胞株生长中的作用。方法：选择不同浓度（0、50、100、200ng／m1）TRAIL作用于人胃癌细胞株BGC-823和HGC-2748h后，用酸性磷酸酶法测定各组细胞株生长抑制情况，确定TRAIL的有效浓度：取有效浓度的TRAIL处理人胃癌BGC-823和HGC-27细胞株48h后，分别用Westem印迹法和RT—PCR检测TRAIL对DAP3蛋白和DAP3mRNA表达的影响。结果：①酸性磷酸酶法测得100ng／mlTRAIL可有效抑制人胃癌细胞株BGC-823和HGC-27生长。②Western印迹法未能在人胃癌细胞株BGC-823和HGC-27中测得DAP3表达．而100ng／mlTRAIL处理后48h，在BGC-823和HGC-27中均可测得DAP3表达：同样．RT—PCR法测得100ng／mlTRAIL处理48h后．两株胃癌细胞株中DAP3mRNA表达显著升高。结论：TRAIL能有效抑制人胃癌细胞株BGC-823和HGC-27的生长．其机制与促进DAP3表达有关。

Tumor necrosis factor-related apoptosis-induced ligand inhibits the growth of human gastric cancer cell lines via enhancing death-associated protein 3 expression

Objectives To explore the role of death-associated protein3(DAP3) in the inhibitory effects of tumor necrosis factor (TNF)-related apoptosis-induced ligand (TRAIL) on the growth of human gastric cancer cell lines. Methods Inhibitory effects of various concentrations of TRAIL on the growth of human gastric cancer cell lines, BGC-823 and HGC-27 were determined by acid phosphatase assay. Meanwhile, the expression of DAP3 before and after TRAIL treatment on RNA and protein level was detected by RT-PCR and Western blot respectively. Results TRAIL can significantly inhibit the growth of BGC-823 and HGC-27 at the concentration of 100 ng/ml. DAP3 expression became detectable after exposure to TRAIL at the concentration of 100 ng/ml. Accordingly, the mRNA expression of DAP3 was elevated after TRAIL treatment. Conclusions TRAIL could significantly inhibit the growth of BGC-823 and HGC-27 human gastric cancer cell lines via enhancing the expression of DAP3.