融合蛋白D2C-SN对登革2型病毒增殖的影响

目的：观察登革2型病毒衣壳蛋白（D2C）与葡萄球菌核酸酶（SN）融合蛋白在哺乳动物细胞中对登革2型病毒增殖的影响。方法：通过基因重组构建可在哺乳动物细胞中表达融合蛋白D2C-SN的重组质粒pc／D2C-SN，同步构建pc／D2C-SN用作对照。病毒感染BHK21细胞后，通过阳离子转染试剂将重组质粒导入感染细胞，在此基础上评价融合蛋白D2C-SN抗登革病毒感染的治疗效果。结果：融合蛋白D2C-SN能够在哺乳动物细胞中表达，对宿主细胞没有明显的毒性；与正常BHK21细胞相比较，可导致病毒感染性滴度降到原来的1／60—1／12。结论：融合蛋白D2C-SN在细胞水平能够有效抑制登革病毒的增殖。有可能成为潜在的抗登革病毒感染的治疗性药物。

Effect of D2C-SN fusion protein on dengue 2 virus multiplication

Objective:To observe the therapeutic effect of D2C-SN fusion proteins against dengue 2 virus infection in vivo.Methods:The eukaryotic vector expressing pc/D2C-SN was constructed by genetic recombination,and pc/D2C-SN~* was simultaneously constructed as control.The recombinant plasmids were then transfected into BHK-21 cells pre-infected with wild dengue 2 virus,and the effect on dengue virus multiplication was evaluated by examining the production of infectious virions.Results: The intracellular expressed fusion protein D2C-SN had no cytotoxicity on BHK cells and resulted in a reduction by about 12-to 60-fold in infectious titers compared with wild dengue 2 virus infection.Conclusion:The fusion proteins D2C-SN could obviously inhibit the multiplication of dengue 2 virus in mammalian cells,and may become a potential drug against infection of dengue virus.