| 粗茎秦艽组织培养及再生植株 |
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| 引用本文： | 孟玉玲,贾敬芬. 粗茎秦艽组织培养及再生植株[J]. 中草药, 1995, 26(10): 537-539,560 |
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| 作者姓名： | 孟玉玲 贾敬芬 |
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| 作者单位： | 兰州大学生物系!730000 |
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| 摘 要： | 用粉茎秦艽Gentianacrassicaulis子叶和下胚轴为材料。在MS＋2mg／L2，4－D＋0．5mg／LBA的培养基上进行培养，子叶及下胚轴的出愈率为100％。愈伤组织继代培养于MS＋0．5mg／L2，4-D＋0．5mg／LBA＋0．5mg／LNAA＋500mg／LLH＋3倍MS有机物和维生素的培养基上生长旺盛。愈伤组织转入分化培养基MS＋1mg／LNAA＋2mg／LBA＋3mg／LZT＋3mg／LGA＋500mg／LLH＋6％蔗糖＋3倍MS有机物和维生素＋3倍FeSO4（Na2－EDTA）上培养，其中40％形成体细胞胚。体细胞胚在无激素MS培养基上发育成苗。
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| 关 键 词： | 秦艽 组织培养 体细胞胚胎发生 再生植株 |
| 收稿时间： | 1994-05-16 |
Tissue Culture of Thickstemen Gentian(Gentiana crassicaulis)and Plant Regeneration Through Somatic Embryogenesis |
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| Meng Yuling and Jia Jingfen. Tissue Culture of Thickstemen Gentian(Gentiana crassicaulis)and Plant Regeneration Through Somatic Embryogenesis[J]. Chinese Traditional and Herbal Drugs, 1995, 26(10): 537-539,560 |
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| Authors: | Meng Yuling and Jia Jingfen |
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| Affiliation: | Department of Biology, Lanzhou University, Lanzhou 730000;Department of Biology, Lanzhou University, Lanzhou 730000 |
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| Abstract: | Callus of Gentiana crassicaulis was initiated from hypocotyl and cotyledon on MS medium supplemented with 2mg/L 2.4-D and 0.5 mg/L BA.Induction frequencies of callus were 100%.Somatic embryos were formed on MS medium containing 2mg/L BA,3mg/L ZT, 1mg/L NAA,3mg/L GA,three time MS organics and vitamins,6% sucrose and 500 mg/L LH and three time FeSO4 (Na2EDTA) .40% Callus produced somatic embryos.It developed into complete plants on hormon-free MS medium. |
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