| 人白细胞介素13cDNA的克隆及序列测定 |
| |
| 引用本文： | 范灵芝,贾松惠.人白细胞介素13cDNA的克隆及序列测定[J].中国免疫学杂志,1997,13(4):222-222. |
| |
| 作者姓名： | 范灵芝 贾松惠 |
| |
| 作者单位： | 中国预防医学科学院病毒所病毒基因工程国家重点实验室，第四军医大学病理教研室 |
| |
| 摘 要： | 用反转录－多聚酶链反应（ＲＴ－ＰＣＲ）技术从中国人外周血淋巴细胞中克隆了ＩＬ－１３ｃＤＮＡ，序列测定表明克隆的ＩＬ－１３ｃＤＮＡ含成熟的ＩＬ－１３蛋白全部编码，且存在编码第９８位Ｇｌｎ的密码子ＣＡＧ，这为进一步表达ＩＬ－１３并深入探究功能奠定了基础。
|
| 关 键 词： | 白细胞介素13 基因克隆 序列测定 聚合酶链反应 |
CLONE AND SEQUENCE OF HUMAN INTERLEUKIN 13 cDNA |
| |
| Fan Lingzhi,Yang Xinke,Hou Yunde.National Laboratory of Molecular Virology and Genetic Engineering.CLONE AND SEQUENCE OF HUMAN INTERLEUKIN 13 cDNA[J].Chinese Journal of Immunology,1997,13(4):222-222. |
| |
| Authors: | Fan Lingzhi Yang Xinke Hou YundeNational Laboratory of Molecular Virology and Genetic Engineering |
| |
| Institution: | Fan Lingzhi,Yang Xinke,Hou Yunde.National Laboratory of Molecular Virology and Genetic Engineering,Institute of Virology,Chinese Academy Preventive Medicine,Beijing 100052 Jia Songhui,Zhao Lijun,Liu Yanfan.Department of Pathology,the Forth Military Med |
| |
| Abstract: | The hIL 13 cDNA was cloned from the activated human lymphocytes by RT PCR with one and half pairs of oligonucleotid primers.The amplified products of hIL 13 cDNA was cloned into pGEM 3zf( ) vector.The recombinant plasmid pGEM 3zf( )/IL 13 was charaterized with restriction enzyme digestion and sequenced with dideoxynucleotide termination method.The results show that the gene encoding full hIL 13 cDNA mature protein was obtained. |
| |
| Keywords: | IL 13 Polymerase chain reaction Gene clone Sequencing |
| 本文献已被 CNKI 维普 等数据库收录！ |
