脆性组氨酸三联体在喉癌中的表达

目的探讨脆性组氨酸三联体(fragile histidine triad,FHIT)在喉鳞状细胞癌(简称喉癌)中的表达,并分析其与p53、增殖细胞核抗原(proliferating cell nuclear antigen,PCNA)以及临床病理学特征的关系。方法采用免疫组化SP法检测60例喉鳞癌、25例癌旁组织、25例喉角化症和20例声带息肉标本中FHIT表达,使用同样方法检测实验组60例喉癌中突变型p53和PCNA的表达水平,结合喉癌患者的临床病理学特征进行分析。结果①在喉癌、癌旁组织和喉角化症中均检测到不同程度FHIT缺失,低表达,在喉癌中最高,缺失,低表达率分别为61．7%(37/60)、36．0% (9/25)、32．0%(8/25)；声带息肉中未见FHIT缺失/低表达(0/20)；②喉癌中FHIT缺失/低表达率高于癌旁组织、喉角化症和声带息肉(x~2=4．682,P=0．030；x~2=6．243,P＜0．01 3：x~2=22．946,P=0．000)；癌旁组织和喉角化症中FHIT缺失/低表达率均明显高于声带息肉(x~2=6．891,P=0．009；x~2=5．749,P=0．017)；喉角化症中FHIT缺失/低表达率略低于癌旁组织,但差异无统计学意义(x~2=0．089,P=0．765)；③FHIT表达与喉癌患者年龄、性别、肿瘤的原发部位、颈部淋巴结转移情况、临床分期及组织病理学分级均无明显相关性(P＞0．05)；④FHIT与p53表达无明显相关性(g =0．103,P=0．434)；FHIT与PCNA表达具有明显负相关性(g=-0．413,P=0．001)。结论FHIT缺失,低表达在喉癌的发生中可能起到重要作用,是喉癌发生的早期事件,可能与肿瘤的恶性增殖有关。

Expression of fragile histidine triad in laryngeal carcinoma

OBJECTIVE To investigate the role of FHIT in the pathogenesis and progression of laryngeal squamous cell carcinoma(LSCC).The correlation of the FHIT expression with the clinicopathologic characte ristics,including p53 expression and PCNA(proliferating cell nuclear antigen)expression,was also studied. METHODS The FHIT expression in LSCC specimen of 60 patients was studied with immunohistochemistry method.In addition,25 adjacent tissues,25 laryngeal keratosis tissues and 20 vocal cord polyp tissues were served as internal controls,vocal cord polyp tissues were regarded as noncancerous epithelium, laryngeal keratosis tissues as precancerous condition. RESULTS Abnormal FHIT expression(markedly reduced or lost)was found in 61.7% of LSCC tissues. No abnormal FHIT expression was found in vocal cord polyp tissues.Alterations of the FHIT expression had also been found in laryngeal keratosis tissues (32.0%)and adjacent tissues(36.0%).The results showed statistically significant difference between the expression of FHIT in LSCC tissues and laryngeal keratosis tissues,adjacent tissues.No correlation was found between FHIT expression and clinical characte ristics,including age,gender,tumor primary sites,lymph node status,stage grouping and pathological grade. The significant association was not found between FHIT and p53 alterations.A significant correlation was found between negative FHIT expression and the high PCNA expression.CONCLUSION Loss of FHIT protein expression may play an important role in the early-stage of the malignant transformation of LSCC.FHIT alteration is associated with a high proliferation that results in an aggressive behavior.