银杏内酯B对实验性动脉粥样硬化的抗氧化作用研究

目的：探讨银杏内酯B对动脉粥样硬化（AS）大鼠的抗氧化作用.方法：采用高脂饲料喂养和维生素D3腹腔注射的方法建立大鼠AS模型,分为正常组、模型组、阳性组（辛伐他汀5mg· kg-1）、银杏内酯B三个剂量组（8,4,2 mg·kg-1）,每组10只.灌胃给药4周后,各组取主动脉,光镜下观察病理改变,进行病变分级评分;取血清,测定血脂水平、超氧化物歧化酶（SOD）活力和丙二醛（MDA）含量.结果：银杏内酯B能改善AS大鼠的主动脉病变,高剂量组与模型组比较有统计学意义（P＜0.05）.银杏内酯B高、中、低剂量可分别降低AS大鼠血清三酰甘油（TG）、总胆固醇（TC）、低密度脂蛋白胆固醇（LDL-C）水平,银杏内酯B高、中剂量可显著升高AS大鼠血清高密度酯蛋白胆固醇（HDL-C）水平,与模型组比较,差异均有统计学意义（P＜0.05或0.01）.银杏内酯B各剂量能显著升高SOD活性,降低AS大鼠血清MDA含量,与模型组相比,差异有统计学意义（P＜0.05或0.01）.结论：银杏内酯B的抗AS作用可能与增强SOD活性、减少MDA含量、减轻氧自由基的损伤有关.

Anti oxidative Effect of Ginkgolide B against Atherosclerosis in Rats

Objective：To investigate the anti-oxidative effect of ginkgolide B （GB） against atherosclerosis （AS） in rats.Methods：AS rat model was established by the administration of high-fat diet and vitamin D3 （ip）.The rats were divided into 6 groups randomly （n =10）,namely the normal group,model group,positive group （simvastatin,5 mg· kg-1） and GB group respectively with high,medium and low dose （8,4 and 2 mg · kg-1 accordingly）.After the 4-week treatment,the aortic pathologic changes were observed under a microscopy and graded.The serum samples were collected to determine the lipid levels,SOD activities and MDA contents.Results：GB could improve the severity of aortic pathology in AS rats,and the pathologic grade of GB group with high dose showed significant difference when compared with that of the model group （P ＜ 0.05）.GB with the different doses （8,4 and 2 mg· kg-1） could markedly decrease the levels of TG （1.10 ± 0.17,1.09 ± 0.26 and 1.20 ± 0.30 mmol · L-1,respectively）,TC （5.43 ± 0.57,5.60 ± 0.90 and 6.02 ± 0.88 mmol· L-1,respectively） and LDL-C （3.44 ± 0.50,3.35 ± 0.92 and 3.80 ± 0.79 mmol · L-1,respectively） in serum of AS rats （P ＜ 0.05 or P ＜ 0.01）.GB （8 and 4 mag · kg-1） could significantly increase HDL-C level （1.03 ± 0.29 and 0.96 ± 0.27 mmol · L-1,respectively） in serum of AS rats （P ＜ 0.05）.Meanwhile,GB （8,4 and 2 mg · kg-1） could significantly increase SOD activity （107.36 ± 19.80,103.82 ± 28.51 and 92.25 ± 18.40 U · ml-1,respectively） and reduce MDA content （8.13 ± 0.96,8.32 ± 1.60 and 9.76 ± 1.33 nmol · ml-1,respectively） in serum of AS rats when compared with those of the model group （P ＜ 0.05 or P ＜ 0.01）.Conclusion：Ginkgolide B shows promising prophylactic effect in AS rats,which may be relative with the actions on SOD activity and MDA content.