| 哮喘大鼠肺组织Toll样受体3和9的表达及布地奈德的影响 |
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| 引用本文： | 方丽,陈培英,童夏生,陈琪,范广民,王恩智.哮喘大鼠肺组织Toll样受体3和9的表达及布地奈德的影响[J].中国现代应用药学,2014,31(7):805-809. |
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| 作者姓名： | 方丽 陈培英 童夏生 陈琪 范广民 王恩智 |
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| 作者单位： | 浙江省诸暨市人民医院儿科,浙江省诸暨市人民医院儿科,浙江省台州市中西医结合医院,台州中心医院检验科,台州中心医院病理科,台州市中西医结合医院检验科 |
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| 基金项目： | 温岭市科技局基金资助项目( 2009-2-55) |
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| 摘 要： | 目的 观察哮喘大鼠肺组织中Toll样受体3（TLR3）和TLR9的表达及对布地奈德的影响,探讨TLRs在哮喘炎症机制中的作用。方法 建立大鼠哮喘模型,随机分成哮喘组、对照组和布地奈德组,免疫组织化学法检测肺组织TLR3和TLR9的表达。结果 哮喘组（OD值：0.201±0.034）和布地奈德组（OD值：0.195±0.043）肺组织TLR3的光密度值显著高于对照组（OD值：0.144±0.039）（P〈0.05）,布地奈德组与哮喘组差异无统计学意义。哮喘组（OD值：0.236±0.022）和布地奈德组（OD值：0.231±0.023）肺组织TLR9的光密度值均显著低于对照组（OD值：0.271±0.025）（P〈0.05）;布地奈德组与哮喘组差异无统计学意义;肺组织TLR3和TLR9蛋白的表达水平无显著相关性（n=26,r=0.153）。结论 哮喘大鼠肺组织TLR3蛋白表达升高,TLR9蛋白的表达则反之,TLRs的表达过度或不足可能是哮喘气道炎症的重要原因之一。布地奈德的抗炎作用可能主要不是通过TLR3和TLR9的途径实现。
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| 关 键 词： | 哮喘 Toll样受体 糖皮质激素 免疫组织化学 |
| 收稿时间： | 2013/8/19 0:00:00 |
| 修稿时间： | 2013/12/14 0:00:00 |
Effect of Budesonide on Expression of Toll Like Receptor 3 and 9 in Rat Asthma Model |
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| FANG Li,CHEN Peiying,TONG Xiasheng,CHEN Qi,FAN Guangmin and WANG Enzhi.Effect of Budesonide on Expression of Toll Like Receptor 3 and 9 in Rat Asthma Model[J].The Chinese Journal of Modern Applied Pharmacy,2014,31(7):805-809. |
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| Authors: | FANG Li CHEN Peiying TONG Xiasheng CHEN Qi FAN Guangmin and WANG Enzhi |
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| Institution: | FANG Li, CHEN Peiying, TONG Xiasheng, CHEN Qi, FAN Guangmin, WANG Enzhi(1.Department of Pediatrics, People＇s Hospital of Zhuji, Shaoxing 311800, China, 2.Taizhou Integrated Traditional and Western Medicine Hospital a.Department of Pediatrics, b.Department of Clinical Laboratory, Taizhou 317000, China,＂ 3. Taizhou Central Hospital a.Department of Clinical Laboratory, b.Department of Pathology, Taizhou 318000, China) |
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| Abstract: | OBJECTIVE To investigate the potential roles of Toll like receptor（TLR） in pathogenesis of asthma inflammation, and the expression of TLR 3 and 9 in rat asthma model. METHODS The rats were randomly and averagely divided into asthma, control and budesonide-treated group. Expressions of TLR3 and TLR9 protein were detected by immunohistochemical method. RESULTS Expressions of TLR3 protein in lung tissure in asthma group（optical density： 0.201±0.034） and in budesonide-treated group（optical density： 0.195±0.043） were significantly higher than that in control group（optical density： 0.144±0.039）（all P〈0.05）. While there was no significant difference of TLR3 protein expression between budesonide-treated and asthma group. Dramaticly, expressions of TLR9 protein in lung tissure in asthma group（0.236±0.022 optical density） and budesonide-treated group（optical density： 0.231±0.023） were significantly lower than that in control group（optical density： 0.271±0.025）（all P〈0.05）. Moreover, there was no significant difference of TLR9 protein expression between in budesonide-treated and asthma group. Furthermore, there was no significant correlation between levels of TLR3 and TLR9 in lung tissue（n=26, r=-0.153,P〉0.05）. CONCLUSION Level of TLR3 increased in asthmatic asthma lung tissue group On the other hand, level of TLR9 decreased. TLRs may act as an important role in asthma exacerbation, and the antiinflammation function of TLRs may not mainly work through TLR3 and TLR9 path way. |
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| Keywords: | asthma Toll like receptor glucocorticoid immunohistochemistry |
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