HPLC法同时测定畲药檵木叶中没食子酸和槲皮素

目的：建立畲药檵木叶中没食子酸和槲皮素的含量测定方法.方法：采用高效液相色谱法测定,色谱柱为Agilent-SBC18（250 mm×4.6mm,5μm）;流动相为甲醇-0.4％磷酸溶液,梯度洗脱;流速1.0 ml·min-1,柱温30℃;检测波长254 nm.结果：没食子酸和槲皮素的线性范围分别为0.094～0.282 μg（r ＝0.999 9）,0.015 ～0.136 μg（r=1.000 0）;平均加样回收率分别为98.97％（RSD=1.74％）,99.05％（RSD=1.84％）.结论：该方法简便易行、准确、重复性好,可用于畲药檵木叶的质量控制.

Simultaneous Determination of Gallic Acid and Quercetin in Traditional She Medicine Folium Loropetali by HPLC

Objective：To establish an HPLC method for the determination of gallic acid and quercetin in traditional She medicine Folium Loropetali.Methods：HPLC was applied with the chromatographic conditions as follows：the chromatographic column was an Agilent-SB C18（250 mm ×4.6 mm,5 μm） at 30℃,methanol-0.4％ phosphoric acid as the mobile phrase with gradient elution,the flow rate was 1.0 ml · min-1 and the detection wavelength was 254nm.Results：The linear range was 0.094-0.282 μg（r =0.999 9） and 0.015-0.136 μg（r =1.000 0）,the average recovery was 98.97％ （RSD =1.74％） and 99.05％ （RSD =1.84％）,respectively.Conclusion：The method is simple,accurate and repeatable,and suitable for the determination of gallic acid and quercetin in traditional She medicine Folium Loropetali.