激活素A对人肝星状细胞系LX-2细胞增殖的影响

目的观察激活素A对人肝星状细胞(hepatic stellate cell,HSC)系LX-2细胞增殖的影响。方法体外培养人HSC LX-2。设正常对照组和激活素A干预组,其中激活素A干预组分为9个浓度梯度,药物终浓度分别为1、2.5、5、10、20、40、80、160、320μg/L,采用CCK-8法检测激活素A对细胞增殖的影响;再选取25、50、100、200、300μg/L5个浓度分别作用24、48、72 h,观察激活素A对细胞增殖的影响。结果激活素A可刺激LX-2细胞增殖,1～2.5μg/L浓度范围内作用轻微(P>0.05),而80～320μg/L的激活素A作用明显增强(P<0.01),且在细胞指数生长期对刺激细胞增殖有时间浓度依赖性。结论激活素A可通过刺激HSC增殖,参与肝纤维化的形成。

Effects of Activin A on Proliferation of Hepatic Stellate Cell Line LX-2

Objective To observe the effects of activin A on hepatic stellate cell(HSC)line LX-2 proliferation.Methods Cultured HSC LX-2 cells were divided into two groups:normal control group and activin A intervention group.The activin A intervention group contained different concentrations of activin A 1,2.5,5,10,20,40,80,160,320 μg/L respectively.Cell proliferation was measured by cell counting kit-8(CCK-8).Then five concentrations of activin A(25,50,100,200,300 μg/L) were selected to intervene LX-2 cells at 24,48,72 h respectively,cell proliferation was also measured by CCK-8.Results Data from CCK-8 showed that activin A could promote LX-2 cells proliferation at concentrations from 1 μg/L to 320 μg/L.Slight effects were observed at concentrations of 1～2.5 μg/L(P>0.05),but significantly increased was shown from 80 μg/L to 320 μg/L(P<0.01).In cell index vegetal period,activin A showed time concentration-dependent stimulation effect.Conclusion Activin A may contribute to hepatic fibrogenesis by stimulating the proliferation of HSC.