| 支气管肺泡灌洗术在哮喘小鼠模型中的应用 |
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| 引用本文： | 酆孟洁,邱晨,刘雯雯.支气管肺泡灌洗术在哮喘小鼠模型中的应用[J].国际检验医学杂志,2012,33(19):2305-2306. |
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| 作者姓名： | 酆孟洁 邱晨 刘雯雯 |
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| 作者单位： | 暨南大学第二临床医学院/深圳市人民医院呼吸内科,广东深圳,518020 |
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| 基金项目： | 广东省自然科学基金资助项目(No.10151802001000004) |
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| 摘 要： | 目的 探讨哮喘小鼠模型支气管肺泡灌洗术标准化操作方法.方法 构建哮喘小鼠模型,使用24 G静脉留置针对小鼠全肺进行支气管肺泡灌洗术,共3次,每次0.8 mL,反复抽注3次.支气管肺泡灌洗液(BALF)离心后取细胞沉淀进行涂片HE染色检查.结果 每只小鼠可顺利回收1.8～2.1 mL BALF,回收率75%～87.5%;哮喘小鼠BALF涂片可见大量嗜酸性粒细胞.结论 采用该方法可成功收集哮喘小鼠模型BALF,可推广应用于其他疾病小鼠模型.
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| 关 键 词： | 哮喘 插管法 气管内 气管切开术 小鼠 支气管肺泡灌洗液 |
Application of broncho-alveolar lavage in murine model of asthma |
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| Feng MengJie
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Qiu Chen
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Liu Wenwen.Application of broncho-alveolar lavage in murine model of asthma[J].International Journal of Laboratory Medicine,2012,33(19):2305-2306. |
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| Authors: | Feng MengJie Qiu Chen Liu Wenwen |
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| Institution: | (Department of Respiratory Diseases,Second Clinical Medical College of Jinan University/Shenzhen People’s Hospital,Shenzhen,Guangdong 518020,China) |
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| Abstract: | Objective To summarize a standard operating procedure(SOP) to gain broncho-alveolar lavage fluid(BALF) in murine model of asthma.Methods The murine model of asthma was generated.Broncho-alveolar lavage fluid(BALF) was taken by injecting saline solution through 24 G superficial vein catheter into murine airways,and slowly redrew the BALF into the connected syringe.0.8 mL normal saline was used in every lavage,and the procedure was repeated 3 times.A mouse was lavaged three times.The collected BALF was centrifuged,and the resuspended cell pellet was used to perform cytospins.Results 1.8-2.1 mL BALF was collected in every mouse,the recovery rate was 75%-87.5%.There were a lot of eosinophils in BALF of asthma murine model.Conclusion The SOP could provide a reliable and easy method to collect BALF from mice. |
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| Keywords: | asthma intubation intratracheal tracheotomy mice bronchoalveolar lavage fluid |
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