Identification of highly conserved loci by genome painting |
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| Authors: | Timothy W. Houseal Joseph A. Cook William S. Modi David W. Hale |
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| Affiliation: | (1) Integrated Genetics, One Mountain Road, 01701-9322 Framingham, MA, USA;(2) University of Alaska Fairbanks, 99775-1200 Fairbanks, AK, USA;(3) Biological Carcinogenesis Development Program, Program Resources, Inc./DynCorp, National Cancer Institute, Frederick Cancer Research and Development Center, 21702-1201 Frederick, MD, USA;(4) Department of Biology, Yale University, 06520-8104 New Haven, CT, USA |
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| Abstract: | Fluorescencein situ hybridization was used to identify patterns of DNA similarity among the genomes of several rodent taxa. Total genomic or Cot-1 DNAs were used as hybridization probes against metaphase preparations across different taxonomic levels, including three species ofMicrotus (suborder Sciurognathi),Mus musculus (suborder Sciurognathi) andCtenomys steinbachi (suborder Hystricognathi). The hybridization patterns ofMus orPeromyscus (sciurognath) DNA toMus metaphases, which were consistent with what is known of the satellite sequences in these species, demonstrated the efficacy of this approach for molecular cytogenetics and evolutionary biology. Additional hybridizations to chromosomes ofCtenomys orMicrotus identified loci consisting of highly conserved DNA sequences. This approach has proved useful in investigating genome homologies across divergent rodent lineages. Chromosome microdissection can be used to characterize these regions further. |
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| Keywords: | fluorescencein situ hybridization genomic DNA molecular evolution repetitive DNA rodent |
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