| 印迹细胞学检查方法在角膜移植排斥反应诊断中的应用 |
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| 引用本文： | 周鸿雁,谷树严,贾卉,李勤,张文松. 印迹细胞学检查方法在角膜移植排斥反应诊断中的应用[J]. 中国实验诊断学, 2002, 6(1): 18-19 |
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| 作者姓名： | 周鸿雁 谷树严 贾卉 李勤 张文松 |
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| 作者单位： | 1. 吉林大学中日联谊医院眼科,吉林,长春,130031
2. 吉林大学第二医院眼科,吉林,长春,130041 |
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| 摘 要： | 目的 探讨印迹细胞学检查方法在诊断角膜移植排斥反应中的应用。方法 用印迹细胞学 (impressioncytology ,IC)检查法追踪观察大鼠角膜移植排斥反应细胞学变化规律 ,以正常大鼠角膜印迹细胞学检查结果为对照组。结果 角膜移植出现排斥反应时 ,见杯状细胞的逐渐消失及上皮细胞的形态学破坏。正常大鼠角膜杯状细胞始终存在。排斥反应鼠印迹细胞学标本中可见不同数量的炎细胞。结论 印迹细胞学检查方法是一种简便而有效的实验室检查方法 ,可应用于预防和诊断角膜移植排斥反应
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| 关 键 词： | 细胞学检查 角膜移植 动物模型 印迹细胞学 |
| 文章编号： | 1007-4287(2002)06-0018-02 |
| 修稿时间： | 2001-10-29 |
The application of impression cytology detection method in keratoplasty reject diagnosis |
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| ZHOU Hong-yan,GU Shu-yan,JIA Hui,et al.. The application of impression cytology detection method in keratoplasty reject diagnosis[J]. Chinese Journal of Laboratory Diagnosis, 2002, 6(1): 18-19 |
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| Authors: | ZHOU Hong-yan GU Shu-yan JIA Hui et al. |
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| Abstract: | Objective To study The application of impression cytology(IC) detection Methods in keratoplasty reject diagnosis. Methods The technology of IC was used to run after the cytology transformation after the operation . We take the normal rat cornea as control; We observe the IC samples of three human corneas in keratoplasty reject. Results IC check result: The outcome of IC displayed that in the allograft reject rats, the goblet cells extincted gradually and the epithelial cells endured morphology destructions. The goblet cells existed in the normal cornea for all the time. Conclusions The technology of IC is a convenient and operative laboratory examination. It can be used to diagnosis corneal graft reject. |
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| Keywords: | cytology detection keratoplasty animal model impression cytology |
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