一种新型结核分枝杆菌感染快速检测方法的应用

目的探讨酶联免疫斑点试验（ELISPOT）快速诊断结核病的价值。方法选取15例确诊为活动性肺结核的患者和100例入境人员的外周抗凝全血标本，采用ELISPOT检测试剂盒（TSPOT-TB）测定结核杆菌特异抗原刺激反应的效应T淋巴细胞数量。结果TSPOT-TB检测结核病的敏感性为93．3％，特异性为100．0％，阳性预测值为100．0％，阴性预测值为81．8％。采用该方法检测的结核潜伏感染率与病例来源呈密切相关，而与年龄和性别无相关性。结论TSPOT-TB的敏感性和特异性均较高，可用于结核感染的快速检测，但无法判断结核的活动性或潜伏感染。

Application of a new method for the rapid detection of Mycobacterium tuberculosis

Objective To study the value of the enzyme-linked immunosorbent spot test(ELISPOT) in the rapid diagnosis of tuberculosis. Methods 100 anti-coagulated peripheral whole blood samples from entry-exit personnel and 15 samples from tuberculosis patients being diagnosed in Huashan hospital were detected by ELISPOT kit (T SPOT-TB) to determine the amount of tuberculosis antigen specific effecter T lymphocytes. Results The sensitivity and specificity of ELISPOT test were 93.3% and 100.0% respectively. The positive predictive value and negative predictive value were 100.0% and 81.8% respectively. The positive rate of T SPOT-TB was correlated with the source of cases, but not with sex or age. Conclusions Both the sensitivity and the specificity of T SPOT-TB kit are relatively high, which is applicable for rapid detection of tuberculosis infection. This technology can not differentiate between active tuberculosis infection and latent infection.