成人急性白血病细胞三色流式细胞术的免疫分型

目的:探讨成人急性白血病(AL)细胞三色流式细胞术免疫分型的特点.方法:采用CD45/SSC双参数散点图设门方法、三色流式细胞术对197例急性白血病细胞进行免疫表型分析.结果:急性淋系白血病细胞均表达淋系抗原,46.7%和11.1%的成人ALL细胞表达CD13和CD33;急性髓系白血病细胞均表达髓系抗原,M3中只有1例表达CD34,HLA-DR均无表达;同时AML中有12例表达淋系抗原CD7,21例表达CD10;197例急性白血病中有11例(占5.6%)为杂舍型急性白血病(HAL),其中有6例B/M型.结论:CD45/SSC设门方法能排除骨髓中正常细胞的干扰,免疫表型的检测和研究对急性白血病的分型和明确诊断有帮助.

Immunophenotyping of 197 adults acute leukemias by three color flow cytometry

Objective:To evaluate the specificity of three-color flow cytometry in adult acute leukemia immunophenotyping.Methods:Immunophenotyping of one hundred and ninety seven adults with acute leukemia was performed by three-color flow cytometry analysis using CD45/SSC gating.Results:Lymphoblastic antigens in all acute lymphoblastic leukemia cases were expressed,in which CD13 and CD33 were 46.7% and 11.1%,respectively;myeloid antigens in all acute myeloid leukemia cases were expressed,and CD34 was expressed in only one of M3 and no expression of HLA-DR was found in all of M3;CD7 and CD34 expression were twelve and twenty one in AML,respectively;Among one hundred and ninety seven cases of AL,5.6% was HAL, six cases of which were B/M.Conclusion:Gating of CD45/SSC can eliminated effect of normal cells on blasts in bone marrow,immunophenotyping results were useful to subtyping and diagnosis of acute leukemia.