运用Micro-PET技术观察环维黄杨星D抗大鼠心肌缺血的实验研究

目的 应用Micro-PET扫描技术，观察环维黄杨星D对大鼠心肌缺血的保护作用。方法 取SD雄性大鼠24只，随机分为4组:假手术组、模型组、地高辛阳性药组和环维黄杨星D组。除假手术组外，其余各组大鼠均采用结扎冠状动脉法制备心肌缺血模型。分别在建模后第1天和给药第7天尾静脉注射18FFDG显像剂，注射1h后，通过Micro-PET扫描，测量大鼠心肌标准摄取值（Standard uptake value，SUV）评价心肌活力，通过感兴趣区域(VOI)勾画评价大鼠心肌梗死率的变化。结果 与模型组比较，阳性药组、环维黄杨星D组SUV明显升高（P<0.05~0.01），心肌梗死率明显降低（P<0.05~0.01）。结论 借助于Micro-PET分子影响手段可在活体条件下评价环维黄杨星D对心肌缺血大鼠的保护作用。 

Protective effects of cyclovirobuxine D on myocardial ischemic in rats by Micro-PET

OBJECTIVE To evaluate the protective effects of Cyclovirobuxine D on myocardial ischemic rats by the method of Micro-PET scanning technology. METHODS All male rats were randomly divided into sham group， model group， digoxin group and Cyclovirobuxine D group. The myocardial ischemic models were established in all groups except sham group. The imaging agent 18FFDG was injected intravenously into tail in rats on the first day of the model and 7th day during medication. The Standard Uptake Value(SUV) of the heart was measured by the method of Micro-PET scanning technology. The changes of myocardial infarction rate were evaluated by Volume of Interest (VOI) sketch. RESULTS The levels of SUV of the heart were improved and the myocardial infarction rates were reduced in digoxin group and Cyclovirobuxine D group(P<0.05~0.01)， compared with the model group. CONCLUSION Cyclovirobuxine D has protective effects on myocardial ischemic rats， which can be concluded by the Micro-PET imaging technology in living conditions of rats.