维吾尔族血小板抗原1~5、15系统基因多态性分析 |
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引用本文: | 吐尔洪·克维尔,左宏莉,方春富,杨勇,文国新. 维吾尔族血小板抗原1~5、15系统基因多态性分析[J]. 新疆医科大学学报, 2006, 29(12): 1128-1130 |
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作者姓名: | 吐尔洪·克维尔 左宏莉 方春富 杨勇 文国新 |
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作者单位: | 新疆维吾尔自治区血液中心输血研究所,新疆,乌鲁木齐,830000 |
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基金项目: | 乌鲁木齐市科学研究与技术开发计划项目 |
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摘 要: | 目的:调查研究与血小板输注无效关系密切的人类血小板抗原(human platelet antigen,HPA)1~5及15系统基因在维吾尔族人群中的遗传多态性;分析民族差异,确定各系统临床意义,从而建立已知基因型血小板供者库。方法:采用聚合酶链一序列特异性引物技术(PCR—SSP)对153例无血缘关系的纯维吾尔族血样进行了HPA基因分型。结果:维吾尔族HPA-1a、2a、3a、4a、5a、15a基因频率分别是0.9118、0.8889、0.5458、0.9935、0.9216、0.4412,维吾尔族HPA-1b、2b、3b、4b、5b、15b基因频率分别是0.0882、0.1111、0.4542、0.0065、0.0784、0.5588,维吾尔族HPA基因频率与汉族人群相比,HPA-1a,1b、3a、3b、5a、5b基因频率差异具有统计学意义(P〈0.05);与西藏藏族人群相比,HPA-3a、3b、5b基因频率差异具有统计学意义(P〈O.05)。结论:维吾尔族人群中HPA1~5、15系统12个等位基因都可以检测到。在不同的个体中它们以纯合子或杂合子状态表达,但未检测到纯合子1b或纯合子4b型个体。HPA-1、2、3、4、5、15系统均具有多态性,其中HPA-3、15系统具有高度多态性,这使免疫暴露的机会增加。维吾尔族人群与汉族人群的HPA-1a、1b、3a、3b、5a、5b基因频率存在明显差异,与藏族人群的HPA-3a、3b、5b基因频率存在明显差异。
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关 键 词: | 人类血小板抗原 基因频率 血小板抗体 |
文章编号: | 1009-5551(2006)12-1128-03 |
收稿时间: | 2006-09-23 |
修稿时间: | 2006-09-23 |
Analysis on the genetic polymorphism of human platelet antigen (HPA)1~5 and 15 systems in Uighurs |
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Abstract: | Objective: To investigate the genetic polymorphism of human platelet antigen(HPA)1 through 5 and 15 systems in Uighurs,which are closely related to refractoriness to platelet transfusion therapy.To analyze the variance in different nationalities and determine their clinical significance for the purpose of establishing a typed platelet donor bank.Methods: PCR-SSP was used to HPA genotyping among 153 unrelated Uighur volunteer donors.Results: Gene frequencies for HPA-1a,2a,3a,4a,5a,and 15a were found to be 0.911 8,0.888 9,0.545 8,0.993 5 and 0.921 6,0.441 2,respectively.Gene frequencies for HPA-1b,2b,3b,4b,5b,15b were 0.088 2,0.111 1,0.454 2,0.006 5,0.078 4,0.558 8,respectively.Compared with Han Chinese,the HPA-1a,1b,3a,3b,5a and 5b gene frequencies of Uighurs had significant differences(P<0.05).Marked differences were also observed in the HPA-3a,3b and 5b gene frequencies between Tibets and Uighurs(P<0.05).Conclusion: Among the Uighur population,we have detected 12 alleles of the(HPA)1 through 5 and 15 systems.In different individuals,they are expressed either as homozygote or heterozygote.However,HPA-1b and 4b alleles are not detected in the population.These results suggest high polymorphism in the HPA 1~5 and 15 systems among Uighurs.Among them,HPA-3 and HPA-15 show a high polymorphism,increasing the incidence for immunological exposure.Compared with Han Chinese,the HPA-1a,1b,3a,3b,5a and 5b gene frequencies of Uighurs have significant differences.Marked differences were also observed in the HPA-3a,3b and 5b gene frequencies between Tibets and Uighurs. |
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Keywords: | human platelet antigen gene frequencies platelet antibody |
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