| 用BA和ABC技术对角膜感染痢疾F_(2a)菌豚鼠眼泪特异抗体的观测 |
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| 引用本文： | 初连瑞,孔祥英,陈志华,苏新. 用BA和ABC技术对角膜感染痢疾F_(2a)菌豚鼠眼泪特异抗体的观测[J]. 军事医学科学院院刊, 1986, 0(1) |
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| 作者姓名： | 初连瑞 孔祥英 陈志华 苏新 |
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| 作者单位： | 军事医学科学院微生物流行病研究所(初连瑞，孔祥英，陈志华)，军事医学科学院微生物流行病研究所(苏新) |
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| 摘 要： | 本文报告了用自制的N-羟基琥珀酰亚胺生物素酯和抗生物素蛋白建立检测豚鼠IgA,IgG的微量BA和ABC技术,其敏感度达0.3～0.5ng/ml,并将BA法用于观测角膜感染痢疾F_(2a)菌后,豚鼠眼泪特异IgA,IgG水平。结果显示,感染后15天患侧眼泪特异IgA、IgG抗体阳性滴度(GMT)分别达400~(-1)和200~(-1)。感染后35天眼泪特异抗体水平明显下降。这些结果,为痢疾局部感染免疫关系的研究提供了方法和依据。
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| 关 键 词： | 微量BA ABC技术 观测泪液特异IgA IgG抗体 痢疾菌 |
DETECTION OF SPECIFIC ANTIBODIES IN TEAR OF GUINEA PIGS INFECTED WITH SHIGELLA FLEXNERI 2a WITH MICRO BA AND MICRO ABC ELISA |
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| Chu Lianrui,Kong Xiangying,Chen Zhihua,Su Xin Institute of Microbiology and Epidemiology,Academy of Military Medical Sciences. DETECTION OF SPECIFIC ANTIBODIES IN TEAR OF GUINEA PIGS INFECTED WITH SHIGELLA FLEXNERI 2a WITH MICRO BA AND MICRO ABC ELISA[J]. Bulletin of the Academy of Military Medical Sciences, 1986, 0(1) |
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| Authors: | Chu Lianrui Kong Xiangying Chen Zhihua Su Xin Institute of Microbiology Epidemiology Academy of Military Medical Sciences |
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| Abstract: | Specific antibodies against Shigella flexneri 2a in the tear of guinea pigs were det-ected by using micro BA ELISA and ABG ELISA.Fifteen days after infection ofcornea,the GMT of specific IgA and IgG antibodies was 400 and 200 respectively.Furthermore,the levels of specific antibodies dropped markedly 35 days after infection.The results show that this method may be used in the study of infection and immunityin shigellosis. |
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| Keywords: | BA ABG ELISA tear specific IgA IgG antibodies Shigella |
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