| 梯度离心法分离食管Langerhans细胞方法初探 |
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| 引用本文： | 文小岗,张云汉,晏梅,付保进. 梯度离心法分离食管Langerhans细胞方法初探[J]. 郑州大学学报(医学版), 1997, 0(3) |
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| 作者姓名： | 文小岗 张云汉 晏梅 付保进 |
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| 作者单位： | 河南医科大学第一附属医院病理科 |
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| 基金项目： | 国家“八五”科技攻关项目,河南省重大科技攻关项目 |
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| 摘 要： | 以甲基泛影酸钠和聚蔗糖配制不连续的密度梯度离心液，分离纯化食管粘膜Ｌａｎｇｅｒｈａｎｓ细胞（Ｌａｎｇｅｒｈａｎｓｃｅｌ，ＬＣ），并以ＣＤ１ａ抗原阳性表达作为ＬＣ判断标准。结果显示：此法可将正常食管粘膜ＬＣ的浓度平均由分离前的１．２％提高至分离后的２１％。提示：该法能从食管粘膜中分离出一定纯度的ＬＣ。
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| 关 键 词： | Langerhans细胞；食管；离心 |
Preliminary exploration of the method to isolate esophageal Langerhans cells with discontinuous density gradient centrifugation |
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| Wen Xiaogang,Zhang Yunhan,Yan Mei,Fu Baojin. Preliminary exploration of the method to isolate esophageal Langerhans cells with discontinuous density gradient centrifugation[J]. Journal of Zhengzhou University: Med Sci, 1997, 0(3) |
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| Authors: | Wen Xiaogang Zhang Yunhan Yan Mei Fu Baojin |
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| Affiliation: | Wen Xiaogang,Zhang Yunhan,Yan Mei,Fu Baojin Department of Pathology,the First Affiliated Hospital,Henan Medical University,Henan Key Laboratory for Tumor Pathology,Zhengzhou 450052 |
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| Abstract: | In Human esophageal Langerhans cells were isolated with discontinuous gradient centrifugation solution prepared with Ficall 400 and sodium metricote.The isolated LCs can be averagely enriched from 1.24% in normal esophageal epidermal before isolation to 21% in the acquired cell suspension after isolation by using this method.It is concluded that by using this method a certain degree of purity of LCs can be isolated from esophageal epidermal. |
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| Keywords: | Langerhans cell esophageal isolate. |
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