姜黄素对胰腺癌细胞株生长的抑制作用及可能机制

目的研究姜黄素对胰腺癌细胞增殖的体外抑制作用．探讨其抗癌作用的分子生物学机制。方法MTT法、Annexln V-FITC／PI双染法、细胞周期分析法、透射电镜等方法检测姜黄素对Pan02细胞生长和凋亡的影响．RT—PCR检测姜黄素对Pan02细胞Bax，Bcl-2，COX2，Bak．Survfvin mRNA的表达。结果(1)姜黄素抑制Pan02细胞生长呈量效关系；(2)姜黄素处理组细胞周期发生改变．G2／M期细胞比例增多、S期细胞比例减少；(3)姜黄素处理组细胞发生凋亡．表现为凋亡细胞比例增多，形态上出现明显细胞凋亡：(4)姜黄素处理组Pan02细胞Bcl-2和Bak mRNA表达水平显著下降．COX-2．Bax mRNA表达水平显著升高，Survivin mRNA的表达水平没有变化。结论姜黄素能够抑制Pan02细胞增殖，它可改变细胞周期、诱导细胞凋亡．Bcl-2家族而不是IAP家族(至少不是Survivin)参与了Pan02细胞的凋亡过程。

Inhibitory effect of Curcumin on pancreatic carcinoma cell line and possible mechanisms

Objective To study the inhibitory effect of Curcumin on cell proliferation of pancreatic carcinoma in vitro, and discuss the molecular biologic mechanism of its anti-carcinoma function. Methods Influence of Curcumin on proliferation and apoptosis of Pan02 cells was evaluated by MTT, Annexin V-FITC/PI double staining, cell cycle-phase analysis and transmission electron microscopy; the expression of Bax, Bcl-2, COX-2. Bak and Survivin mRNA in Pan02 cell treated with Curcumin was assessed by RT-PCR. Results (1)The inhibitory effect of Curcumin on Pan02 cell proliferation was found to be in a dose-dependent manner; (2)Cell cycle change was observed in the group treated with Curcumin; the percentage of cells in G2/M stage increased, and that in S stage decreased; (3)Cell apoptosis was observed in the group treated with Curcumin, which was represented by the increased percentage of apoptotic cells and presence of cells with marked morphological change; (4) The expression of Bcl-2 and Bak mRNA in Pan02 cells treated with Curcumin down-regulated significantly, while the level of COX-2 and Bax mRNA up-regulated significantly. There was no change in Survivin mRNA expression. Conclusions Curcumin inhibited the proliferation of Pan02 cells by changing cell cycle and inducing apoptosis. It is the BCL-2 family, not the IAP family (at least not Survivin) ,that participates in the apoptosis of Pan02 cells.