RP-HPLC-DAD法同时测定吴茱萸汤中7个生物碱和2个黄酮苷含量(英文)

建立反相高效液相色谱-二极管阵列检测 (RP-HPLC-DAD) 法同时测定经典名方吴茱萸汤中7个生物碱 (去氢吴茱萸碱、10-羟基吴茱萸次碱、吴茱萸碱、吴茱萸次碱、1-甲基-2-正壬基-4(1H)喹诺酮、吴茱萸卡品碱和二氢吴茱萸卡品碱) 和2个黄酮苷 (异鼠李素-7-O-芸香糖苷和香叶木素-7-O-β-D-吡喃葡萄糖苷)的含量。RP-HPLC分离应用反相C18 色谱柱, 甲醇-1%醋酸水溶液梯度洗脱, 流速为1.0 mL/min, 检测波长为300 nm。上述9个分析物达到基线分离, 标准曲线线性关系良好(r2>0.9941), 日内和日间精密度和准确度符合分析方法学要求, 加样回收率为90.13%-102.48% (RSDs<3.6%)。经方法学验证, 所建立的方法适用于吴茱萸汤物质基础的质量控制。

Simultaneous determination of seven alkaloids and two flavonoid glycosides in Wuzhuyu decoction by RP-HPLC-DAD

A reversed-phase high-performance liquid chromatography(RP-HPLC) method with diode array detector was established to simultaneously determine nine bioactive compounds in Wuzhuyu decoction,namely dehydroevodiamine,isorhamnetin-7-Orutinoside,10-hydroxyrutaecarpine,diosmetin-7-O-β-D-glucopyranoside,evodiamine,rutaecarpine,1-methyl-2-n-nonyl-4(1H) quinolone,evocarpine,and dihydroevocarpine.The RP-HPLC assay was performed on a reversed-phase C18 column with a gradient elution.The mobile phases consisted of methanol and water containing 1%(v/v) acetic acid.The flow rate was 1.0 mL/min and the detection wavelength was set at 300 nm.All calibration curves showed good linearity(r2>0.9941) within test ranges.The results of intra-day precision,inter-day precision,and accuracy were satisfactory.The overall recoveries were in the range of 90.13%-102.48%.The method was successfully applied to quantify nine bioactive compounds in six samples.Baseline resolution for all nine major compounds was achieved.This method could be used as a suitable quality control assay for Wuzhuyu decoction.