双吲哚碱bromotopsentin的抗氧化及抗癌活性

目的对一种由海绵Spongosoritessp.提取的双吲哚碱bromotopsentin(BSM)的抗氧化及抗癌活性进行研究。方法通过DPPH、TEAC等一系列与抗氧化活性相关联的分析方法,分析了BSM的抗氧化活性,并通过细胞毒性和抗增殖实验分析BSM对人宫颈癌细胞(HeLa)及人肺癌细胞(A549)生长的抑制作用。结果BSM对稳定自由基1,1-二苯基苦基苯肼(DPPH)的清除活性超过抗坏血酸的相应活性,其IC50值为48.9μmol.L-1;用6-羟基-2,5,7,8-四甲基苯并二氢吡喃-2-羧酸(Trolox)等效抗氧化能力(TEAC)方法测定BSM总的抗氧化能力大约为1.2,高于抗坏血酸的1.04。BSM对HeLa细胞及A549细胞的生长具有抑制作用,其IC50分别为2.3、24.5μmol.L-1。结论BSM有良好的抗氧化能力,同时对HeLa细胞及A549细胞有较强的细胞毒性作用。

Antioxidant and anticancer activities of a bisindole alkaloid: bromotopsentin

Objective To investigate the antioxidant and anticancer activities of bromotopsentin (BSM), which is a bisindole alkaloid isolated from marine sponge Spongosorites sp. Method The free radical scavenging (DPPH assay) and 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox) equivalent antioxidant capacity (TEAC) were performed to evaluate the antioxidant activity of BSM, and cytotoxicity assay and anti-proliferation assay were performed to evaluate the inhibition effects of BSM on the growth of human cervical carcinoma (HeLa) cells and human lung carcinoma (A549) cells. Result It was found that BSM showed stronger scavenging activity on the stable free radical than L-ascorbic acid with IC50 value of 48.9 μmol.L^-1. TEAC value meaning a total antioxidant capacity of BSM was about 1.2, which was also better than the TEAC value 1.04 of L-ascorbic acid. On the other hand, BSM could also inhibit the growth of the human carcinoma HeLa cells and A549 cells, and their IC50 was 2.3 μmol.L^-1 and 24.5 μmol.L^-1, respectively. Conclusion BSM has a perfect antioxidant activity, and it also has great cytotoxic effect on HeLa cells and A549 cells.