| 重氮乙酰丝氨酸诱导大鼠胰腺腺泡细胞癌癌前病变及其K-ras基因突变的实验研究 |
| |
| 引用本文： | 徐峰,曹伟,寿楠海,姜希宏,徐克森. 重氮乙酰丝氨酸诱导大鼠胰腺腺泡细胞癌癌前病变及其K-ras基因突变的实验研究[J]. 癌变．畸变．突变, 1999, 0(2) |
| |
| 作者姓名： | 徐峰 曹伟 寿楠海 姜希宏 徐克森 |
| |
| 作者单位： | 山东医科大学附属医院普外科(徐峰，寿楠海，姜希宏，徐克森)，山东医科大学基础医学院药理学教研室!济南250012(曹伟) |
| |
| 摘 要： | 建立大鼠胰腺腺泡细胞癌癌前病变的动物模型，了解该癌前病变及Ｋｒａｓ 基因突变的情况 雄性Ｗｉｓｔａｒ 大鼠单次腹腔注射重氮乙酰丝氨酸３０ｍｇ／Ｋｇ 体重，４ 个月后常规取胰腺组织行光镜和电镜检查，并应用ＰＣＲＳＳＣＰ法检测Ｋｒａｓ 基因突变情况 大鼠胰腺腺泡细胞癌癌前病变发生率１００％ ，Ｋｒａｓ 基因突变率４２－１ ％ 。重氮乙酰丝氨酸可成功诱导大鼠胰腺腺泡细胞癌癌前病变，Ｋｒａｓ 基因突变在此过程中属早期事件。
|
| 关 键 词： | 重氮乙酰丝氨酸 胰腺肿瘤 Kras基因 |
THE EXPERIMENTAL STUDY OF PRECANCEROUS LESION OF PANCREATIC ACINAR CELLULAR CANCER IN RATS INDUCED BY AZASERINE AND THE DETECTION OF K RAS MUTATION |
| |
| Abstract: | To set up the animal model of precancerous lesion of rat's pancreatic acinar cellular cancer, learn its biological feature and K ras gene code 12 mutation. Male Wistar rats were injected Azaserine 30mg/kg(ip). 4 months later, rats' pancreas were autopsied and K ras gene code 12 mutation were detected by PCR SSCP. The incidence of precancreous lesion of rats' pancreatic acinar cellular cancer was 42.1%.Azaserine could induce precancerous lesion of rats' pancreatic acinar cancer successfully, K ras gene code 12 mutation was early change during this process. |
| |
| Keywords: | Azaserine Pancreatic neoplasm K ras gene |
| 本文献已被 CNKI 等数据库收录！ |
