Brief counting method of airborne Cryptomeria japonica pollen by a combination of fluorescence antibody staining and flow cytometry] |
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| Authors: | Y Takahashi S Yoshimura H Masago K Inouye T Shirai T Nagoya M Sakaguchi S Inouye S Katagiri |
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| Affiliation: | Yamagata Prefectural Institute of Public Health. |
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| Abstract: | Airborne pollens collected in a pollen collector (Virtual Impactor) was treated with a fluorescein isothiocyanate-labeled monoclonal antibody (KW-S10) which was strictly specific to Japanese cedar pollen antigen (Cry j I). Flow cytometric analysis revealed that the intensity of fluorescence of the pollen samples treated with the antibody was greater than that of non-treated reference pollen or the antibody treated Hinoki-cypress pollen. By use of this method, it may be possible to display the airborne pollen concentration within 20 min after sampling. |
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