金标免疫渗滤法诊断试剂盒诊断血吸虫病的价值研究

目的　评价金标免疫渗滤法诊断试剂盒（DIGFA-kit）用于血吸虫病血清流行病学调查的价值。方法　采用DIGFA与ELISA单盲平行检测各期血吸虫病病人血清、非流行区人群血清、疫区人群血清和血吸虫病已控制地区人群血清共2304份。结果　DIGFA检测血吸虫抗体的敏感性为96.8%，对非流行区健康人群的特异性为100%；该法检测疫区居民血清508份，抗体阳性检出率为35.6%，在Kato法粪检阳性的81人中，其中抗体阳性79人，阳性符合率为97.5%；检测血吸虫病已控制地区人群血清，抗体阳性率为4.6%；根据DIGFA的敏感性和对非流行区人群的特异性，计算其Youdens指数为0.926。上述各项结果与ELISA比较，差异无显著性（P>0.05）。结论　DIGFA与ELISA有相似的敏感性和特异性，且方法简便、快速，胶体金标记物制备容易，试剂稳定，运送方便，在现场和临床诊断更具有优越性。

EVALUATION OF DOT IMMUNOGOLD FILTRATION ASSAY KIT FOR RAPID DIAGNOSIS OF SCHIST OSOMIASIS

Objective To evaluate the ability of Dot Immunogold Filtr ation Assay Kit (DIGFA-Kit) to detect schistosomiasis in the epidemiological in vestigation Methods A total of 2 304 sera from patients with schis tosomiasis at different stages, population in non-endemic areas, population in endemic areas and in control areas were detected by DIGFA-Kit and ELISA. Results The sensitivity of DIGFA-Kit was 96.8% and its specificity wa s 100% to health population in non-endemic area. Of 508 peoples in endemic are as, 79 were positive DIGFA-Kit while 81 were found to have Schistosoma infe ction by Kato-Katz. The coincidence rate was 97.5%. Positive rate was 4.6% in p opulation from schistosomiasis controlled areas. Youdens index for DIGFA was 0 .926. There w as no significant difference in comparison with ELISA(P>0.05). Conclu sion DIGFA-Kit held the same sensitivity and specificity as ELISA. Moreo ver, this kit is simple, rapid and easy to handle and its reagents are stable , easy to prepare and convenient to transfer. Those indicated that DIGFA-Kit ha s potential diagnostic value for schistosomiasis in the field or in the hospital .