| 肺炎结合疫苗PPS14-OVA、PPS14-C3d在小鼠脾脏的定位及补体对定位的影响 |
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| 引用本文： | 毕晓云,潘恩山,吴志东,曹玉华,胡勇.肺炎结合疫苗PPS14-OVA、PPS14-C3d在小鼠脾脏的定位及补体对定位的影响[J].免疫学杂志,2010(2). |
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| 作者姓名： | 毕晓云 潘恩山 吴志东 曹玉华 胡勇 |
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| 作者单位： | 广州经济技术开发区医院检验科;南方医科大学中医外科教研室;广州精达医学科技有限公司; |
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| 摘 要： | 目的检测血清型14型肺炎球菌荚膜多糖(PPS14)结合疫苗PPS14-OVA(卵清蛋白)、PPS14-C3d(补体)免疫小鼠后在脾脏细胞中的定位,以及补体对其定位的影响。方法PPS14-OVA、PPS14-C3d经皮下或静脉注射,免疫正常小鼠和眼镜蛇毒因子(CVF)预处理的小鼠,应用免疫荧光技术检测脾脏冰冻切片中的PPS14及各种免疫细胞。结果正常小鼠免疫PPS14-OVA后,PPS14-OVA定位于脾脏;1 h后与脾脏边缘区B细胞有结合;2 h后出现在淋巴滤泡中;6 h后全部转移至淋巴滤泡,并与滤泡B细胞和树突状细胞结合,与巨噬细胞和T细胞没有结合。而CVF预处理的小鼠,PPS14-OVA不定位于脾脏。PPS14-C3d在脾脏中的定位不受CVF处理的影响。结论 PPS14-OVA在脾脏中的定位需要补体的介导,而PPS14-C3d不需要。
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| 关 键 词： | 补体 PPS14 C3d 冰冻切片 免疫荧光 |
Localization of conjugate vaccines PPS14-OVA and PPS14-C3d in mouse spleen and effects of complement on localization of the conjugate vaccines |
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| BI Xiaoyun,PAN Enshan,WU Zhidong,CAO Yuhua,HU Yong.Localization of conjugate vaccines PPS14-OVA and PPS14-C3d in mouse spleen and effects of complement on localization of the conjugate vaccines[J].Immunological Journal,2010(2). |
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| Authors: | BI Xiaoyun PAN Enshan WU Zhidong CAO Yuhua HU Yong |
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| Institution: | BI Xiaoyun,PAN Enshan,WU Zhidong,CAO Yuhua,HU Yong Department of Clinical Laboratory,Guangzhou Economic & Technology Development District Hospital,Guangzhou 510730,China |
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| Abstract: | To investigate the localization of conjugate vaccines consisting of serotype 14 pneumococcal capsular polysaccharide (PPS14) coupled to either ovalbumin(OVA) or C3d in mouse spleen cells,and to evaluate the role of complement in localization of the conjugate vaccines.Mice were immunized with PPS14-OVA or PPS14-C3d by either subcutaneous or intravenous injection,while some mice pretreated with cobra venom factor(CVF).Immunofluorescence was used to examine the frozen sections of spleen from all mice for delec... |
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| Keywords: | Complement PPS14 C3d Frozen section Immunofluorescence |
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