Production of bifunctional monoclonal antibodies to human IgG and horseradish peroxidase and their utilization for testing anti-HIV antibodies |
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| Authors: | Yu. S. Massino L. L. Sukhanova E. A. Kizim O. L. Segal M. B. Smirnova V. V. Monakhov G. I. Kolyaskina A. D. Dmitriev |
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| Affiliation: | (1) Laboratory of Conditioned Reflex Neurochemistry, Institute of Higher Nervous Activity and Neurophysiology, Russian Academy of Sciences, Moscow;(2) Laboratory of Viral Genome Cloning, Institute of Viral Preparations, Russian Academy of Medical Sciences, Moscow;(3) Laboratory of Neuroimmunology, Mental Health Center, Russian Academy of Medical Sciences, Moscow |
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| Abstract: | Bispecific antibodies are purified from ascitic fluid by successive affinity chromatography on HRP-Sepharose and IgG-Sepharose. The ability of the affinity purified bispecific antibodies to bind human IgG and horseradish peroxidase is demonstrated in the immunoenzyme assay (identification of anti-HIV antibodies. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 117, № 3, pp. 291–293, March, 1994 Presented by M. E. Vartanyan Member of the Russian Academy of Medical Sciences |
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| Keywords: | quadroma bispecific antibodies human IgG peroxidase anti-HIV antibodies |
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