Enhancement of phagocytosis by dynorphin A in mouse peritoneal macrophages |
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Authors: | Mitsuyuki Ichinose Masatoshi Asai and Masashi Sawada |
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Institution: | a Department of Physiology, Shimane Medical University, Izumo 693, Japan b Department of Physics, Shimane Medical University, Izumo 693, Japan |
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Abstract: | The effects of the opioid peptide dynorphin A (DynA) on phagocytosis in peritoneal macrophages was examined by flow cytometry (FCM). DynA enhanced phagocytosis in a dose-dependent manner. Leucine-enkephalin (Leu-Enk), methionine-enkephalin (Met-Enk), β-neo-endorphin (βNeo-End), DynA(9–17) and DynA(13–17) had no such activity, -Neo-endorphin ( Neo-End), dynorphin B (DynB), DynA(l–13) and DynA(6–17) enhanced phagocytosis less effectively than DynA. Naloxone did not inhibit the enhancement of phagocytosis induced by DynA. Unstimulated control phagocytosis was partially suppressed in Ca2+-free EGTA-containing solution and even in this solution DynA enhanced phagocytosis. However, the enhancement by DynA was suppressed in EGTA- and BAPTA-AM-containing Ca2+-free solution. The present study showed that enhancement of phagocytosis by DynA was independent of extracellular Ca2+ (Ca2+]o) and dependent on intracellular Ca2+ (Ca2+]i). The present results support DynA being one of the mediators from the nervous system that modulates the immune system. |
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Keywords: | Flow cytometry Macrophages Dynorphin A Phagocytosis |
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