紫杉醇在大鼠原位肝移植中的作用及其对细胞毒性T淋巴细胞实验的影响

目的观察紫杉醇在大鼠原位肝移植中的作用及其对细胞毒性T淋巴细胞(CTL)的影响。方法建立Wistar→SD大鼠肝移植的急性排斥模型。将大鼠分为对照组(阻断全肝血流)、急排组(Wistar→SD肝移植)、紫杉醇组(Wistar→SD肝移植,紫杉醇腹腔注射0.75mg·kg-1·d-1,观察生存时间、病理改变、生化指标及CTL、T淋巴细胞凋亡情况。结果紫杉醇组大鼠的术后存活时间高于急排组,(18.2±1.5)dvs(8.8±1.9)d,P0.01)。急排组的HE评分(Banff)高于紫杉醇组(7.80±0.83vs4.80±0.82,P0.01)。紫杉醇组的白蛋白水平高于急排组(P=0.003);紫杉醇组的谷丙转氨酶水平低于急排组(t=25.23,P0.01)。CTL检测中,急排组的特异性杀伤靶细胞的百分率均高于紫杉醇组(P0.01)。急排组的T淋巴细胞的凋亡率低于紫杉醇组(7.16±1.56)%vs(16.47±2.51)%,P0.01]。结论紫杉醇在大鼠肝移植中具有免疫抑制作用,可能通过诱导T淋巴细胞凋亡来抑制T淋巴细胞的活化发挥作用。

Effect of paclitaxol in rat orthotopic liver transplantation and its impact on the CTL

Ojective To observation of the effect of paclitaxol in rat orthotopic liver transplantation and its impact on the cytotoxic T lymphocyte（CTL）.Methods Wistar → SD rat liver transplantation model of acute rejection was established.The rats were divided into three groups：control group（blocking total hepatic blood flow）,acute rejection group（Wistar → SD liver transplantation）,paclitaxel group（Wistar → SD liver transplanta-tion,paclitaxel intraperitoneal injection of 0.75 mg·kg-1·d-1.The survival time,pathological changes,biochemical markers and CTL,T lymphocyte apoptosis were observed.Results The postoperative survival time of paclitaxel group were rats higher than acute rejection group,（18.2±1.5）d vs（8.8±1.9）d,P0.01.HE score（Banff）of acute rejection group is higher than the paclitaxel group,7.80±0.83 vs 4.80±0.82,t=5.67,P0.01.Albumin level in paclitaxel group ranked higher than acute rejection group,P=0.003,while alanine aminotransferase of paclitaxel group were lower than acute rejection group,P0.01.In CTL,percentage of anti-target cell in a-the cute rejection group were higher than the percentage of paclitaxel group,P0.01.Tlymphocyte apoptosis rate of acute rejection group isless than the paclitaxel group,（7.16±1.56）% vs（16.47±2.51）%,0.01.Conclusion P Paclitaxel exerts immunosuppression in rat liver transplantation,by inducing T lymphocyte apoptosis to suppress T lymphocyte activation probably.