CIK细胞体外逆转多药耐药性的研究

目的:研究CIK细胞体外逆转多药耐药(multidrug resistance,MDR)可行性。方法:用人外周血分离出的单个核细胞,在不同细胞因子诱导下培养成细胞因子诱导的杀伤细胞(cytokine-induced killer,CIK)及树突状细胞(DC),然后将其与成熟DC进行共培养,作为效应细胞,以K562敏感株与耐药细胞株为靶细胞进行体外逆转研究。流式细胞术检测靶细胞的P-gp表达及细胞内的ADR相对含量,MTT法测定其细胞毒作用,RT-PCR检测mdr-1基因耐药性逆转表达状况。结果:K562/ADR耐药细胞株ADR含量经效应细胞细胞作用后,细胞内的ADR含量分别提高了13.80%、39.50%,(P<0.01);,P-gp分别降低12.00%、42.69%,(P<0.01),mdr-1基因表达呈下调趋势,效应细胞对靶细胞的细胞毒作用分别达45.8%、78.9%,(P<0.01)。结论:CIK+K562/ADR-DC对P-gp高表达K562/ADR耐药细胞株具有特异性的细胞毒作用,有效提高了细胞内的ADR含量,下调了P-gp、mdr-1表达,使免疫效应细胞体外逆转多药耐药的效果得以显现。

The research of CIK cells reverse multi-drug resistance in vitro

Objective: To explore the feasibility of CIK cells reverse multi-drug resistance in vitro.Methods: PBMNC,derived from peripheral,resistant K562/ADR were induced into CIK and DC with different cytokines,then CIK were co-cultured with mature DC,being the effecter cell in order to do the research of the reverse effect with the susceptible and resistant strains of K562 as target cell.Measure the cytotoxicity with MTT.The P-gp expression of target cells and the concentration of intracellular ADR were detected by...