沂蒙山区3年32例阿尔茨海默病患者淀粉样β蛋白及其前体基因表达量的相关性分析

背景:淀粉样β蛋白沉积是引致阿尔茨海默病的重要原因,淀粉样β蛋白前体基因过度表达或某部分发生突变,可能与阿尔茨海默病的发生有关。目的:观察沂蒙山区阿尔茨海默病与淀粉样β蛋白及其前体基因的关系。设计:病例-对照分析。单位:临沂市人民医院神经内科。对象:选择临沂医专附属临沂市人民医院神经内科2001-01/2003-12住院阿尔茨海默病患者32例,以性别、年龄相当的非颅脑损伤的外科手术老年人30例为对照组(简易精神状态量表评分>27分)。方法:应用ELISA法检测脑脊液淀粉样β蛋白水平,应用荧光定量聚合酶链反应技术检测淀粉样β蛋白前体基因表达量。结果:62例全部进入结果分析。①阿尔茨海默病组脑脊液淀粉样β蛋白水平明显高于对照组(13.63±9.34),(6.75±5.37)μg/L,t=3.354,P<0.01]。②阿尔茨海默病组脑脊液淀粉样β蛋白前体表达量明显高于对照组(1624±298),(1275±269)拷贝/μL,t=4.42,P<0.01]。③阿尔茨海默病组淀粉样β蛋白水平与淀粉样β蛋白前体基因表达量呈正相关(r=0.44,P<0.01)。结论:沂蒙山区阿尔茨海默病患者脑脊液中淀粉样β蛋白及其前体基因表达增高,且淀粉样β蛋白水平越高,其前体基因表达越高,痴呆程度越重。提示阿尔茨海默病与淀粉样β蛋白、淀粉样β蛋白前体密切相关。但淀粉样β蛋白前体表达量在阿尔茨海默病诊断中不具特异性。

Correlation analysis on beta amyloid protein and expression of amyloid precursor protein gene in 32 patients with Alzheimer disease for 3 years in Yimeng mountainous area

BACKGROUND: Deposition of beta-amyloid protein is the main cause of Alzheimer disease. Overexpression of β-amyloid precursor protein gene (βAPP-gene) or mutation of it at certain part can be related with the attack of Alzheimer disease.OBJECTIVE: To observe the relationship of Alzheimer disease and β-amyloid protein with β APP-gene in Yimeng mountainous area.DESIGN: A case-control analysis study.SETTING: Department of Neurology, Linyi Municipal People's Hospital.PARTICIPANTS: Thirty-two Alzheimer disease inpatients were enrolled at the Department of Neurology, Linyi Municipal People's Hospital affiliated to Linyi Medical Professional College between January 2001 and December 2003. Thirty elderly patients who were treated with surgery of noncraniocerebral injury and were of the same sex and the similar age were regarded as the members of the control group score of Mini Mental State Examination (MMSE) was over 27 points].METHODS: Level of β-amyloid protein in cerebrospinal fluid was detected with enzyme-linked immunoadsordent assay (ELISA). Expression of βAPP-gene was measured with the technic of fluorescent quantitative polymerase chain reaction.RESULTS: A total of 62 cases were involved in the result analysis. ①The level of β-amyloid protein in cerebrospinal fluid in the Alzheimer disease group was higher significantly than that in the control group(13.63±9.34), (6.75±5.37)μg/L,t=3.354, P ＜ 0.01]. ② The expression ofβ APP in cerebrospinal fluid in the Alzheimer disease group was higher significantly than that in the control group (1 624±298), (1275±269)copy/μL,t=4.42, P ＜ 0.01]. ③ The level of β-amyloid protein was posit ively correlated with the expression of β APP-gene in the Alzheimer disease group (r=0.44,P＜ 0.01).CONCLUSION: The level of β-amyloid protein and the expression of β APP-gene in cerebrospinal fluid of Alzheimer disease patients in the Yimeng Mountainous area increase. Besides, the higher the level of β-amyloid protein, the higher the expression of β APP-gene is, and the severer the degree of dementia is. It indicates that Alzheimer disease has closely relation with β-amyloid protein and β APP-gene. But the expression of the β APP-gene has no specificity in the diagnosis of Alzheimer disease.