血管内皮生长因子在睾丸生殖细胞肿瘤中的表达

 目的 探讨血管内皮生长因子（VEGF）表达及微血管计数与睾丸生殖细胞肿瘤（TGCT）临床病理特征的关系。方法 42例睾丸生殖细胞肿瘤和10例对照（无肿瘤浸润的去势睾丸组织）标本取自武汉大学人民医院病理科。应用免疫组化方法检测标本q-血管内皮生长因子的表达及微血管计数（MVC），HE染色显示脉管（血管或淋巴管）浸润。结果 肿瘤组VEGF阳性表达率及MVC均显著高于对照组。VEGF表达在各病理类型组间无显著差别（P〉0．05）；随临床分期的进展VEGF表达增强（P〈0．001）。Ⅲ期肿瘤的MVC分别显著高于Ⅰ、Ⅱ期。VEGF表达与MVC之间呈较强正相关（r=0．675）。脉管浸润率随临床分期的增加而增加。但多因素分析显示仅VEGF表达与临床分期相关。结论 血管生成与TGCT的转移相关。VEGF在调节TGCT的血管生成及促进TGCT的临床进展方面可能发挥着重要作用，VEGF可作为反映TCK2T生物学行为的客观指标。

The Expression of Vascular Endothelial Growth Factor in Testicular Germ Cell Tumors

Objective To investigate expression feature of vascular endothelial growth factor (VEGF) in testicular germ cell tumors (TGCT) and its clinical significance.Methods Employing immunohistochemistry, VEGF expression and microvessel counts (MVC) were studied on slides of paraffin-embeded specimens of 42 cases of TGCT with diverse pathological types and stages. Vascular invasion was also detected by hematoxylin and eosin (HE) stains.Results VEGF expression and MVC were both significantly higher in tumor group. The expression levels of VEGF rose with clinical stages development (P< 0.001), but did not differ among various pathologic types (P> 0.05). MVC in stage C was significantly higher than in stage A and B, respectively. VEGF expression was markedly correlated with MVC (r= 0.675,P< 0.001). Vascular invasion rate was higher in clinical progressed stages. But when using multiple regression analysis, only VEGF expression revealed a correlation with clinical stages. Conclusion This study suggested that VEGF performed an important role in angiogenesis and might promote the development of TGCT. VEGF would be a potent indicator for biological characteristic of TGCT.