| 人类细胞周期蛋白D3反义表达质粒的构建 |
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| 引用本文： | 程纯,邵晓轶,张冬梅,刘海鸥.人类细胞周期蛋白D3反义表达质粒的构建[J].南通医学院学报,2004,24(3):240-241,244. |
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| 作者姓名： | 程纯 邵晓轶 张冬梅 刘海鸥 |
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| 作者单位： | 南通医学院微生物学教研室,南通,226001 |
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| 基金项目： | 江苏省高校高新技术产业发展项目(JH02-117) |
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| 摘 要： | 目的：构建人类细胞周期蛋白D3(cyclin D3)反义表达质粒，为今后利用反义核酸阻断技术研究cyclin D3基因的功能提供基础。方法：运用DNA重组技术将人cyclin D3基因反向克隆到真核表达载体pcDNA3．1中，构建成cyclin D3反义表达质粒pcDNA3--ascyclinD 3。结果：通过酶切鉴定及测序分析证实，实验成功构建了人cyclin D3反义表达质粒。结论：cyclin D3反义表达质粒pcDNA3--ascyclin D3的成功构建，为进一步研究cyclin D3的生物学功能及其在肿瘤发生、发展、诊疗和预后中的作用奠定了基础。
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| 关 键 词： | 细胞周期蛋白D3 反义核酸阻断技术 细胞周期 聚合酶链反应 |
| 文章编号： | 1000-2057(2004)03-0240-03 |
Construction of eukaryotic expression plasmid expressing human cyclin D3 antisense gene |
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| CHENG Chun,SHAO Xiaoyi,ZHANG Dongmei,et al.Construction of eukaryotic expression plasmid expressing human cyclin D3 antisense gene[J].ACTA Academiae Medicinae Nantong,2004,24(3):240-241,244. |
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| Authors: | CHENG Chun SHAO Xiaoyi ZHANG Dongmei |
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| Abstract: | Objective:To construct the eukaryotic expression plasmid expressing human cyclin D3 antisense gene to study the function of cyclin D3 gene by means of antisense technology.Methods:PcDNA3-ascyclin D3 plasmid was constructed by inserting human cyclin D3 cDNA reversely to eukaryotic expression vector PCDNA3.1.Results:It was comfirmed by DNA sequence analysis that the expression plasmid of antisense cyclin D3 was successfully constructed.Conculusion:The results of this study lay the foundation for further studying the biological functions of cyclin D3 and its role in the occurrence , development, diagnosis, therapy and prognosis of tumors. |
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| Keywords: | Cyclin D3 Antisense interference technique Cell cycle Polymerase chain reaction |
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