正交试验优选四黄膏提取工艺

目的:优选四黄膏提取工艺。方法:乙醇提取大黄、黄芩,正交试验法考察乙醇浓度、乙醇用量、提取时间、提取次数对大黄素含量的影响;水煎煮提取黄连、黄柏,正交试验法考察水的用量、浸泡时间、煎煮时间、煎煮次数对鞣质含量的影响。结果:大黄、黄芩醇提取最佳工艺条件为:乙醇浓度为60%,用量为药材总量的6倍,提取时间为1h,提取次数为3次;黄连、黄柏水提取最佳工艺条件为:水的用量为药材总量的6倍,浸泡时间为0.5h,煎煮时间为2h,提取次数为3次。结论:采用优选工艺制备四黄膏操作简便,稳定性好,可为工业生产提供理论依据。

Optimization of the Extraction Process of Sihuang Paste

OBJECTIVE： To optimize the extraction process for Sihuang paste. METHODS： The Radix et Rhizorna Rhei, Radix Scutellariae were extracted with ethanol, and the effects of the concentration and dosage of ethanol, and the extracting time, extraction times on the content of emodin were investigated by orthogonal experiment. The Rhizorna coptidis and Cortex Phellodendri were extracted by water decoction method, and the effects of amount of water, the soaking time, the decoction time and decoction times on the content of tannin were investigated by orthogonal experiment. RESULTS： The optimum alcohol extracting condition for Radix et Rhizoma Rhei, Radix Scutellaria was as follows： adding 60% alcohol at a dose of 6 times that of the total medicinal substances and extracting for 1 h/ time for 3 times. The optimum water extracting condition for Rhizorna Coptidis and Cortex Phellodendri was as follows： soaking medicinal substances in water （with the ratio of medicinal substances to water at 1 ： 6） for 0.5 h, decocting for 2 h and extracting for 3 times. CONCLUSION： The optimized methods are simple and stable, which provide theoretic basis for industrial production.