尿激酶联合镁剂治疗大鼠急性脑梗死的实验研究

目的　观察尿激酶溶栓联合硫酸镁神经保护对大鼠急性脑梗死的疗效。方法　应用光化学诱导法建立大鼠大脑中动脉闭塞(MCAO)模型,分别于术后2 h、6 h和10 h 3 个时间点进行干预,每个时间点内再分为生理盐水对照组、尿激酶溶栓组、尿激酶加硫酸镁治疗组,术后24 h观察大鼠神经功能缺损评分及脑梗死体积的变化。结果　MCAO后2 h尿激酶溶栓组神经功能显著改善,梗死体积缩小(与生理盐水对照组相比,P<0.01),尿激酶加硫酸镁治疗组效果更好;MCAO后6 h、10 h尿激酶溶栓组与生理盐水对照组相比无显著性差异(P>0.05),而尿激酶加硫酸镁治疗组的神经功能缺损评分、脑梗死体积与生理盐水对照组及尿激酶溶栓组相比有显著差异(P<0.05或P<0.01)。结论　早期脑梗死特别是2 h内的超早期脑梗死应用尿激酶溶栓有效;加用镁剂进行神经保护可对尿激酶溶栓疗效产生协同作用,并可能扩大脑梗死溶栓治疗的时间窗。

Experimental study of the combinative therapy with urokinase and magnesium on acute cerebral infarction in rats

Objective To investigate the effect of urokinase (UK) thrombolysis in combination with magnesium sulfate as neuroprotective agent on acute cerebral ischemia in tats.Methods The middle cerebral artery occlusion (MCAO) model was induced in rats by photochemical method.The rats were treated at 2 h,6 h and 10 h postocclusion respectively.In each time spot in saline group,urokinase group,urokinase and magnesium sulfate group,neurologic functional score and infarct volume were observed at 24 h after MCAO.Results Urokinase thrombolysis in 2 hours postocclusion was effective in improvement of neurologic deficit score and reduction of the infarct volume significantly (compaired with saline group, P < 0.01 ),UK combined with magnesium had better efficacy.The infarct size and neurologic score failed to show evidently recovery when treated by UK at 6 and 10 hours after MCAO (compaired with control group, P > 0.05 ).The combinative therapy with UK and magnesium could improve the motor function and decrease the infarct volume significantly,Compared with control group and UK group, P < 0.05 or P < 0.01 .Conclusions In acute cerebral infarction,urokinase thrombolysis has significant effect,especially in 3 hours postocclusion.The combination treatment with urokinase and magnesium may prolong the thrombolytic therapeutic time window.