大蒜素对顺铂肾毒性的保护作用

目的：研究大蒜素对顺铂（DDP）导致的人胚肾HEK293细胞氧化损伤的保护作用并探讨其作用机制。方法：体外培养HEK293细胞，MTT法分别测定不同浓度大蒜素和DDP对HEK293细胞生长的影响，以及大蒜素预处理对DDP诱发细胞毒性的影响。进一步将HEK293细胞分为对照组（未加入DDP和大蒜素）、DDP组（20 mg/L）、大蒜素组（2 mg/L）、大蒜素（4 mg/L）+DDP（20 mg/L）组，黄嘌呤氧化酶法检测各组细胞内超氧化物歧化酶（SOD）活力，硫代巴比妥酸法测定细胞内丙二醛（MDA）含量，二硫代二硝基苯甲酸法测定还原型谷胱甘肽（GSH）的含量。结果：与对照组相比，DDP导致细胞存活率显著降低，且呈剂量效应关系（P < 0.01）。大蒜素预处理后，对DDP（20 mg/L）所致细胞存活率的抑制作用有显著改善（P < 0.01）。与DDP组相比，大蒜素能显著抑制DDP所致细胞内MDA含量的升高以及SOD活力和GSH含量的下降（P < 0.01）。结论：大蒜素可拮抗DDP所致HEK293细胞氧化损伤，对顺铂肾毒性具有明显的保护作用。

Protective effects of allicin on cisplatin-induced toxicity in HEK293 kidney cells

OBJECTIVE:To investigate the protective effect of allicin on cisplatin (DDP)-induced oxidative damage in HEK293 cells.METHODS:HEK293 cells were cultured in vitro and organized into different experimental groups:control,DDP treatment,allicin treatment,DDP+allicin treatment.Cell survival and expression of cytotoxicity were determined by using the MTT method.Superoxide dismutase (SOD) activity was examined by the xanthine oxidase method,malondialdehyde (MDA) content by the thiobarbituric acid method and glutathione (GSH) content by the nitrobenzoic acid method.RESULTS:Cell survival rate decreased gradually when treated with DDP alone,and this effect was dose-dependent.After pretreatment with catechin and then with DDP (20 mg/L),the cell survival rate improved significantly (P＜0.01).Allicin treatment significantly increased the intracellular MDA content,but decreased the SOD activity and GSH level compared with that in the DDP control group (P＜0.01).CONCLUSION:Allicin can antagonize DDP-induced oxidative damage in HEK293 cells and has obvious protective effects on DDP-induced toxicity in kidney cells.