精子与肿瘤细胞融合模型的建立

目的：构建一种稳定的精子与肿瘤细胞融合体系，为精子与肿瘤细胞融合研究提供生物模型。方法：采用体外共培养的方式进行精子与HeLa细胞等多种肿瘤细胞的融合实验；采用共聚焦显微镜三维重建成像的方法对精子与肿瘤细胞融合现象进行确证；采用嵌套式培养体系作为融合实验的对照研究；采用自主合成的精子与肿瘤细胞共培养基（STCM）对精子与肿瘤细胞融合模型进行优化。结果：精子与肿瘤细胞在体外可以发生融合，融合事件多发生于共培养后的1.5~2 h，精子与肿瘤细胞融合形成的嵌合型肿瘤细胞呈明显的球形改变，其贴附能力明显下降，通常漂浮于培养中。与RPMI 1640培养基相比较，STCM能够在不影响肿瘤细胞生长的情况下，显著提高精子与肿瘤细胞的融合率（P < 0.05），并且能够加速精子与肿瘤细胞地融合。结论：采用体外共培养的方法，我们建立了精子与肿瘤细胞融合的生物模型。

Establishment of a sperm-tumor cell fusion model

OBJECTIVE: To establish a stable system for sperm and tumor cell fusion and to provide a biological model for sperm and tumor cell fusion investigation. METHODS: Fusion of sperm and tumor cells (e.g. Hela) was carried out by their co-cultivation in vitro. The 3D-confocal microscopy imaging was used to confirm the fusion of both cells. The transwell insert culture system was used as a control for cell fusion experiment. The fusion model was optimized by using the self-synthesized sperm-tumor cell culture medium (STCM). RESULTS: Fusion between the sperm and the tumor cells was observed in vitro which generally occurred in 1.5 to 2 hours after co-cultivation. The chimeric cells generally displayed a spherical type and floated in the medium as a result of significantly reduced attachment ability. Comparison with RPMI 1640 medium,STCM not only disturbed the growth of tumor cells but greatly improved the fusion rates between the two cell types (P < 0.05) and accelerated the fusion to some extent. CONCLUSION: We have established a fusion model of sperm and tumor cells by using co-cultivation of both cells in vitro.