川芎嗪治疗重症急性胰腺炎的实验研究

目的探讨川芎嗪对大鼠重症急性胰腺炎的治疗作用及其机制。方法 30只SD大鼠随机分为假手术组、重症急性胰腺炎组(胰腺炎组)和川芎嗪治疗组(治疗组),每组10只。以L-精氨酸注射法制作大鼠重症急性胰腺炎模型,治疗组大鼠在制模成功后给予川芎嗪治疗,在实验后24 h采集标本,比较不同组大鼠胰腺组织HE染色结果,血清淀粉酶、白介素-6(IL-6)、TNF-α水平及胰腺湿/干比率。结果胰腺炎组和治疗组大鼠血清淀粉酶、IL-6和TNF-α水平均显著高于假手术组,差异有统计学意义(P<0.05),治疗组大鼠血清淀粉酶[(883.75±143.25)U/L vs.(1 031.94±146.59)U/L]、IL-6[(148.65±18.94)pg/L vs.(185.79±17.49)pg/L]和TNF-α[(109.31±123.65)pg/mL vs.(155.79±17.49)pg/mL]均低于胰腺炎组,差异有统计学意义(P<0.05)。治疗组大鼠胰腺湿/干比率(9.8±0.7)低于胰腺炎组(12.5±0.8),差异有统计学意义。结论川芎嗪可通过降低重症急性胰腺炎大鼠的血清淀粉酶、IL-6和TNF-α水平发挥治疗作用。

Experimental study of ligustrazine in the treatment of severe acute pancreatitis

Objective To investigate the effect and the mechanism of ligustrazine in the treatment of severe a-cute pancreatitis (SAP) in rats. Methods Thirty SD rats were randomly divided into sham operation group (n=10), SAP group (n=10),and ligustrazine treatment group (n=10). L-aginine injection method was used to manufacture SAP model in rats. Ligustrazine was used to treat SAP for 24 h in rats. The pathological change of pancreatic tissue,ser-um amylase level,serum interleukin-6 level,TNF-α level and wet/dry ratio among different groups in rats were com-pared. Results Serum amylase level,serum interleukin-6 level and TNF-α level in rats of SAP group and ligustrazine treatment group were significantly higher than those of sham operation group ( P < 0. 05 ) . Serum amylase level [(883.75±143.25)U/L vs. (1031.94 ±146.59)U/L],serum interleukin-6 level [(148.65 ±18.94)pg/L vs. (185.79±17.49)pg/L]andTNF-α level[(109.31±123.65)pg/mLvs.(155.79±17.49)pg/mL]ofligustrazine treatment group were lower than those of SAP group,there being significant differences(P<0. 05). The wet/dry ratio of ligustrazine treatment group ( 9. 8 ± 0. 7 ) was less than that in SAP group ( 12. 5 ± 0. 8 ) , there being significant difference. Conclusion Ligustrazine is effective in treating severe acute pancreatitis in rats by decreasing the level of serum amylase,interleukin-6 and TNF-α.