MMP-2、PTEN、VEGF-C在大肠癌组织中的表达研究

【摘要】〓目的〓探讨大肠癌组织中基质金属蛋白酶-2(MMP-2)、磷酸酶基因(PTEN)、血管表皮生长因子C(VEGF-C)的表达。方法〓应用S-P免疫组化法对50例大肠癌手术切除标本进行抗人MMP-2单克隆抗体和PTEN、VEGF-C克隆抗体免疫组化染色,检测癌组织、癌旁组织区域MMP-2和PTEN、VEGF-C表达, 分析其与大肠癌临床病理特征之间的关系。结果〓大肠癌组织中MMP-2、VEGF-C 等表达明显高于正常大肠黏膜组织,PTEN表达明显低于正常大肠黏膜组织,且比较差异均有统计学意义(P<0.05);大肠癌组织分化程度愈低、临床TNM分期越晚、出现淋巴结或远处转移,则大肠癌组织中PTEN的阳性表达率就越低,而MMP-2、VEGF-C的阳性表达率就越高(P<0.05)。结论〓MMP-2和PTEN、VEGF-C与大肠癌的发生、侵袭和转移具有潜在影响作用,检测MMP-2和PTEN、VEGF-C的表达可作为判断大肠癌病变发展和转移潜能的指标。

Expression and clinical significance of MMP-2, PTEN and VEGF-C in colorectal cancer tissues

【Abstract】〓Objective〓To investigate the expression of matrix metalloproteinase (MMP-2), phosphatase gene (PTEN) and vascular epidermal growth factor C (VEGF-C) in colorectal cancer tissues. Methods〓S-P immunohistochemical method was used to detect the expression of MMP-2, PTEN, MMP-2 and VEGF-C in 50 cases of colorectal carcinoma, and the expression of, PTEN and VEGF-C were detected by immunohistochemical staining. Results〓Expression of VEGF-C and MMP-2 in colorectal carcinoma were significantly higher than that in normal colorectal mucosa tissues(P<0.05). PTEN expression was significantly lower than that in normal colorectal mucosa (P<0.05). PTEN and MMP-2 showed higher expression in lower differentiation degree, later stage of TNM, lymph node metastasis while PTEN showed a lower level of expression. Conclusion〓In the present study,the expression of PTEN and VEGF-C, MMP-2 and MMP-2 showed the regular changes in differentiation degree, TNM stage, lymph node metastasis in colorectal carcinoma tissues, and were probably associated with the development and metastasis of colorectal cancer.