实时荧光定量PCR检测原发性肝癌中CDK4基因的表达

目的：应用实时荧光定量PCR法检测原发性肝细胞癌中CDK4基因的表达。方法：提取手术切除人肝癌和癌旁组织总RNA并逆转录为cDNA。应用实时荧光定量PCR法观察人肝细胞癌组织及癌旁组织中cDK4的表达水平。结果：20例肝细胞癌标本中有18例肿瘤组织中CDK4基因表达明显高于癌旁肝组织，其中7例高出4倍以上。结论：实时荧光定量PCR可以准确定量测定基因的表达；CDK4基因在肝细胞癌的发生、发展中可能起重要作用。

Real-time fluorescence quantitative PCR in detecting CDK4 gene expression in hepatocellular carcinoma

Objective:To quantitatively detect the expression level of CDK4 in primary hepatocellular carcinoma. Methods: The total RNA isolated from human HCC and adjacent liver tissue was reversely transcribed into cDNA. The real timefluorescence quantitative PCR method was used to analyze the expression level of CDK4 gene. Results: The real time fluorescence quantitative PCR method was performed successfully to precisely detect the mRNA level. The expression level of CDK4 gene was higher in tumor tissue than that in para tumor liver tissues in 18 cases of 20 cases of hepatocellular carcinoma (HCC). In 7 cases the expression level of CDK 4 showed 4 times difference between the CDK4 expression level of tumor tissue and that of para tumor liver tissue. Conclusion: The real time fluorescence quantitative PCR is the most precise method to quantitatively detect the mRNA level and can be used in gene expression changes. The CDK4 gene has higher expression in HCC tumor tissue cells than in para tumor liver tissue cells,indicating that it plays an important role in the development of the HCC.