| 一氧化氮及其合酶在小鼠急性肝衰竭模型中的产生及意义 |
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| 引用本文： | 夏秦,石淑仙. 一氧化氮及其合酶在小鼠急性肝衰竭模型中的产生及意义[J]. 华中科技大学学报(医学版), 2002, 31(6): 669-671,674,F003 |
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| 作者姓名： | 夏秦 石淑仙 |
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| 作者单位： | 1. 华中科技大学同济医学院附属同济医院综合科,武汉,430030
2. 华中科技大学同济医学院附属同济医院传染科,武汉,430030 |
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| 摘 要： | 为了探讨NO在急性肝衰竭肝细胞损伤中的作用，将雌性BALB/C小鼠按不同时间分组，建立急性肝衰竭动物模型，检测各组小鼠血清中NO代谢产物NO2^-的产生及iNOS基因在肝组织中的表达。结果发现：动物模型建立后血清NO2^-浓度随时间延长呈上升趋势；经原位杂交检测，正常肝细胞内无iNOSmRNA表达，各模型组可见肝细胞内iNOSmRNA呈不同程度的表达，模型组表达情况与正常组比较有显著性差异。提示过量产生的NO作为机体免疫反应的一部分参与了肝细胞的损害，是急性肝衰竭肝损伤的重要介质之一。
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| 关 键 词： | 一氧化氮 急性肝功能衰竭 诱生型一氧化氮合酶 动物模型 |
Production of Nitric Oxide and Nitric Oxide Synthase in Mice with Acute Hepatic Failure |
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| Xia Qin ,Shi Shuxian General Department. Production of Nitric Oxide and Nitric Oxide Synthase in Mice with Acute Hepatic Failure[J]. Journal of Huazhong University of Science and Technology(Health Sciences), 2002, 31(6): 669-671,674,F003 |
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| Authors: | Xia Qin Shi Shuxian General Department |
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| Affiliation: | Xia Qin 1,Shi Shuxian 2 1 General Department,2 Department of Infectious Diseases,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030 |
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| Abstract: | In order to study the role of nitric oxide (NO) in liver cell injury induced by acute hepatic failure, female BALB/C mice were divided into groups according to different time and animal model of acute hepatic failure was created. Serum NO 2 - and expression of iNOS mRNA in liver cells was detected. The results revealed that in the animal model of acute hepatic injury the concentration of serum NO 2 - was increased time dependently. In situ hybridization analysis showed iNOS mRNA expression was not detectable in the normal liver cells. In each model group, iNOS mRNA was expressed in the liver cells to varying degrees. There was significant difference in iNOS mRNA expression in liver cells among the model groups. It was suggested that excess NO might involve in the liver cell injury and NO is one of the important media in liver injury of acute hepatic failure. |
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| Keywords: | nitric oxide acute hepatic failure inducible nitric oxide synthase |
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