过继转输的肿瘤特异性T杀伤细胞在小鼠体内维持杀伤功能的研究

体外试验从免疫小鼠脾细胞中诱导出对ＦＢＬ－３瘤细胞具有特异性杀伤功能的Ｔ杀伤细胞，转输到同系正常小鼠体内后发现，同时给予肿瘤抗原刺激和７ｄｒＩＬ－２作用，可使受鼠脾细胞获得杀伤ＦＢＬ－３细胞的活性，其杀伤功能与转输的Ｔ杀伤细胞有关，若转输后连续２１ｄ只给予ｒＩＬ－２不能维持体内建立的杀瘤活性，而间断给予肿瘤抗原再刺激，并联合应用短程ｒＩＬ－２，以１４ｄ为１周期，则可长期维持体内的肿瘤特异性杀伤功能

TO MAINTAIN THE ANTITUMOR ACTIVITY OF THE TRANSFERRED TUMOR SPECIFIC CTLS OVER PROLONGED PERIODS OF TIME

Tumor specific cytotoxic T lymphocytes were induced in vitro by stimulation with mitomycin C treated FBL-3and expended by culture for 14 days with low concentration of rIL-2 from spleens of B6 mice immune to FBL-3.The resultant donor T cells were adoptively transferred into syngenetic hosts,and stimulated by an injection of tumor antigen plus a 7 day course of rlL-2,the result show that the experimental mice get a significent cytotoxicity to FBL-3,and it is donor CTLs not host lymphocytes that contribute to this lysis,However,prolonging the course of rlL-2 administration to 21 days did not maintain the specific cytolytic function of donor T cells,By contrast.transferred donor T cells could survive in vivo over prolonged periods of time with maintenance of specific cytotoxicity by intermittent restimulation with tumor antigen plus rIL-2.