正交试验法优选茜草配方颗粒的提取工艺

目的 优选茜草配方颗粒的提取工艺。方法 以干浸膏得率、大叶茜草素和羟基茜草素提取量为评价指标,采用正交试验设计法考察加水倍量、提取次数和提取时间3个因素对提取效果的影响,通过综合评分筛选出茜草的最佳提取工艺。结果 提取次数对试验结果有极显著影响,加水倍量对提取工艺的影响有显著意义,而提取时间对结果无显著影响。综合考虑各因素的影响及生产实际需要,确定的最佳提取工艺为:茜草饮片加入10倍量水,煎煮提取3次,每次1小时。结论 该工艺条件提取充分,稳定可行,对茜草配方颗粒的生产具有一定的指导和参考意义。

Optimization of extraction technology of Rubia cordifolia L.for preparing formula granule by othogonal experiment

ObjectiveTo optimize the extraction process of Rubia cordifolia L.formula granule.MethodsExtraction yields of solid drug,rubimaillin and purpurin were regarded as investigation index,and the amount of water,extraction times and duration on extraction were evaluated by orthogonal experimental design,and the best extraction process was statistically analyzed and screened by multi-index comprehensive evaluation method.ResultsExtraction times had the most notable effect on experimental results,and the amount of water was the secondary notable effect on the results,while the duration of extraction had no notable effects.Considering the effects of three factors and actual production process synthetically,the optimum condition of extraction process was that the decoction pieces were decocted three times in 10 folds water,one hour each time.ConclusionThe optimum extraction process was simple,reasonable and stable,which will have some instruction meaning and reference value to the production of Rubia cordifolia L.formula granule.