阿霉素肾病大鼠α-SMA表达特点及缬沙坦的干预治疗

目的观察阿霉素诱导的肾病模型大鼠肾脏αSMA、FN、LN的表达特点、意义和缬沙坦干预治疗的影响。方法单侧肾切除加重复阿霉素尾静脉注射制作肾病模型,以αSMA、FN、LN等为观察指标,采用免疫组化、病理图像分析和流式细胞检测等方法,进行阳性表达定位、半定量和蛋白表达定量分析。结果阿霉素肾病模型组大鼠肾小管间质αSMA表达明显,阳性细胞率和蛋白表达半定量分析明显高于假手术组(P<0.01),且伴有FN、LN在肾小管间质和肾小球的大量沉积,缬沙坦组明显低于模型组(P<0.01)。结论阿霉素肾病模型中肾小管间质存在细胞表型转化现象,缬沙坦能抑制肾小管间质细胞表型转化,同时,减少FN、LN等细胞外基质的沉积。

Study on the expression of α-SMA in rats with adriamycin-induced nephrothy and intervention of Valsartan on it

Aim To observe the changes on the expression of α-SMA in rats with adriamycin-induced nephrothy and intervention of Valsartan. Methods The model group were made by unilateral nephrectomy with twice-adriamycin injections by caudal vein. Immunohistochemical method, flow cytometry and pathologic image analysis were used to observe the expression of α-SMA, FN and LN in rats. Results The expression of α-SMA was positive in the model group. Rates of positive cell and protein semi-quantitative analysis were more higher than the sham group(P<0.01). FN, LN had over expression in model group, and it expressed slightly in Valsartan group(P<0.01). Conclusion The model group had cell phenotype conversion in renal tubulointerstitium. Valsartan had an effect on inhibiting the expression of α-SMA, FN and LN.